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Volume 2
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Parul Thapar, Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C6-017
BACTERIOCIN INDUCED MILK- A VEHICLE
IN PRESERVING ORAL HEALTH
Parul Thapar
Indira Gandhi National Open University, India
T
ooth decay, also called as dental caries has been ranked at 3rd position by
World Health Organization among all chronic non-communicable diseas-
es. It has been pointed out by W.H.O., that despite of great improvements in
the oral health of population in several countries; dental caries still persists
and has become a global problem. Numerous studies have shown that the as-
sociation of
Streptococcus mutans
and
Streptococcus sobrinus
cause higher
levels of caries. Bacteriocins are potent protein toxins produced by virtually
every bacterial and archeal species. In this study, bacteriocins have been iso-
lated from the species of
Lactobacillus brevis
isolated from curd by centrifu-
gation at 12,000 rpm for 5 min using (RM 12C Microcentrifuge, REMI Motors,
Mumbai, India). The isolated bacteriocins were screened for their antibacte-
rial activity against the isolated species of
Streptococcus mutans
and
Strep-
tococcus sobrinus
using spot on lawn assay. Thus, the isolated bacteriocins
could inhibit the growth of these isolates of dental caries. The bacteriocins
were then optimized using different pH, temperature and time combinations
for their maximum production. Their purification was done using ammonium
sulphate precipitation and centrifugal filtration. The bacteriocins with differ-
ent concentrations (0.05 ml, 0.1 ml, 0.5 ml and 1 ml) were then induced in
1ml of skim milk (Experimental Dairy section, NDRI, Karnal). These were sub-
jected for antibacterial activity against isolates of dental caries using agar
well diffusion assay. They were incubated at 37°C for 24 h to observe for the
presence or absence of zone. Therefore, the bacteriocin induced milk showed
inhibition against caries causing organisms. Thus, bacteriocin induced skim
milk can act as a potent in strengthening of oral health.
Parul Thapar is a research scholar in Dairy Science and
Technology (sp.
Dairy Microbiology
) from Indira Gandhi
National Open University, New Delhi, India. Parul has
publications in national and international journals and
Science magazines. Her publications reflect her research
interests in Food, Health and Microbiology. She has been
awarded by BRICPL Young Scholar Award and Young
Achievers Award.
parul.thapar@yahoo.comBIOGRAPHY