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Euro Diabetes Congress 2018, Global Vaccines Congress 2018
& Food Science Conference 2018 Archives of General Internal Medicine | ISSN: 2591-7951| Volume 2
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
&
&
NUTRITION, FOOD SCIENCE & TECHNOLOGY
International Conference on
DIABETES, ENDOCRINOLOGY & NURSING MANAGEMENT
World Congress on
VACCINES & VACCINATION
3rd Global Congress on
Dalia Adel Abdulhalim Hassan, Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C6-016
PARAOXONASE-1 GENE Q192R AND
L55M POLYMORPHISMS AND RISK OF
CARDIOVASCULAR DISEASE IN EGYPTIAN
PATIENTS WITH TYPE 2 DIABETES
MELLITUS
Background: Diabetes mellitus (DM) is a chronic life-threatening disease; peo-
ple with type 2 diabetes mellitus (T2DM) are more likely to develop cardiovas-
cular disease (CVD) than people without diabetes. Increased oxidative stress
or an impaired antioxidant defense mechanism may play a crucial role in the
onset and progression of atherosclerosis. Recently, Paraoxonase−1 (PON1)
which accounts for most of the antioxidant effect of high density lipoprotein
(HDL) cholesterol has been presented as a potential therapeutic agent against
atherosclerosis development. Allele frequencies for PON1 gene that influence
enzyme concentration as well as activity differs greatly among ethnic groups
and data from several studies showed ethnic variations in the interpretation
of CVD associated with PON1 polymorphisms. In this work, we investigated
PON1 Q192R and L55M polymorphisms in Egyptian patients with type 2 diabe-
tes mellitus and its association with CVD.
Methods: The study included 184 subjects classified into 3 groups; T2DM,
T2DM + CVD, and healthy controls. PON1 polymorphisms were genotyped by
real-time PCR and PON1 concentration was assayed in serum by ELISA (en-
zyme linked immune-sorbent assay).
Results: Genotype and allele frequencies of Q192R were significantly differ-
ent between controls and diabetic patients. Frequency of QQ genotype was
significantly higher in healthy controls, while QR and RR genotypes were sig-
nificantly higher in diabetic patients (p=0.02). Frequency of 55LL and LM gen-
otypes were significantly higher in patients than in controls (p=0.009). Q192R
polymorphism associated with CVD in our diabetic patients (p=0.01) and with
low serum PON1 concentration (p=0.04). Multiple logistic regression analysis
revealed significant correlations between 192R and other independent CVD
risk factors.
Conclusion: Our findings support that PON1 192R and 55 L alleles are associ-
ated with T2DM. Q192R polymorphism is associated with CVD and lower se-
rum enzyme concentration and might represents a novel risk factor for CVD in
Egyptian patients with T2DM.
Biography
Dalia Adel Abdulhalim Hassan is an associate pro-
fessor of clinical and chemical pathology, medical
division, National Research Centre, Cairo, Egypt.
She completed M.B., ., MSc., MD. Clinical &
Chemical Pathology, and also Faculty of Medicine
in Cairo University. Her subspecialty in fields of dia-
betes, obesity, hematology, molecular biology and
stem cell transplantation. And her clinical activities
in 20 years of clinical and laboratory experiences.
Worked in peripheral blood stem cell transplanta-
tion and B.M.T. unit in Manial specialized hospital,
faculty of medicine, Cairo University from 1997-
2004. Routine laboratory and molecular work in
the clinical and chemical pathology department of
medical division, National Research Centre from
2004- till now. Involved in a lot of Research Activi-
ties and projects in fields of Diabetes, Obesity, oste-
oporosis, oncology, stem cells and renal diseases.
Dalia Adel Abdulhalim Hassan
National Research Centre, Egypt