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J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
Anti-obesity activities of xanthorrhizol in 3T3-L1 adipocytes
Seok Fang Oon
University of Putra Malaysia, Malaysia
A
ccording to the World Health Organization (WHO), at least
2.8 million people die every year due to overweight or
obesity. The increased obesity prevalence has caused major
health problems such as cardiovascular diseases and diabetes.
Although several anti-obesity drugs have been developed, they
are limiteddue toadverse side effects such as stroke,myocardial
infarction, and depression. These circumstances have increased
the demand for effective and safe anti-obesity agents. Previous
studies demonstrated that xanthorrhizol (XNT) reduced the
levels of serum free fatty acid and triglyceride
in vivo
, but the
detailed anti-obesity activities are yet to be reported. Thus,
this study aims to evaluate the abilities of XNT to impede
adipogenesis, stimulate lipolysis, and its related lipolytic
mechanisms employing 3T3-L1 adipocytes. Adipogenesis was
examined by glycerol-3-phosphate dehydrogenase (GPDH)
activity, whilst lipolysis was investigated by quantifying the
glycerol amount. The mechanisms involved were further
evaluated by protein analysis of leptin and insulin. Statistical
significance was established by one-way ANOVA, where p<0.05
and 0.01 were considered statistically significant. In this study,
XNT decreased GPDH activity in a dose-dependent manner
from 3.125 to 12.5 µg/mL. The highest GPDH inhibition and
glycerol release was 47.74±1.36% and 45.37±1.43% (p<0.05),
respectively. Interestingly, XNT-treated adipocytes produced
leptin at levels that were two times higher than control (p<0.05)
and induced a 64.04±1.73% reduction in insulin expression
(p<0.01). These results revealed that XNT may suppress
adipogenesis and stimulate lipolysis through regulation of leptin
and insulin expression in the adipocytes. In this conference, I
will discuss the anti-obesity activities of xanthorrhizol and how
it works based on our recent protein analysis.
Speaker Biography
Seok Fang Oon has her expertise in investigating the potential health effects of natural
products. She is doing her PhD research in the anti-obesity activities of xanthorrhizol in
University of Putra Malaysia. She has worked as a Senior Research Officer for two years
in the project development of antihypertensive and anti-diabetic natural products. Her
main research interests include the biological effects of natural products and herbal
treatment in hypertension, obesity, diabetes, hyperlipidermia, and cancer
e:
seokfang@live.com