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J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
Hepatoprotective activity of aqueous seed extract of
Nigella sativa
against highly active antiretroviral
therapy induced hepatotoxicity in rats
Kissi Mudie
Ethiopian Public Health Institute, Ethiopia
Background:
Liver is a metabolically active organ responsible
for many vital life functions. It performs many activities that are
critical for survival. Due to its important activities, the liver is
exposed to many insults and is one of the body’s organs most
subject to injury. Despite tremendous advances in modern
medicine, there are hardly any reliable drugs that protect the
liver from damage and/or help in regeneration of hepatic cell.
It is, therefore, necessary to search for effective and safe herbal
drugs for the treatment of liver disease to replace currently
used drugs of doubtful efficacy and safety.
Aim:
Theaimofthisstudywastoinvestigatethehepatoprotective
activity of aqueous extract of
Nigella sativa
seed in highly active
antiretroviral therapy (Lamivudine, Zidovudine and Efavirenz)
administered rats.
Materials & Methods:
Sixty rats weighed between 150-
200 g were randomly divided into six groups and each group
comprised of ten rats. Rats in group I were administered with
distilled water. Rats in group II were administered with highly
active antiretroviral therapy only. Rats in groups III - VI were
administered 100, 200, 400 and 800 mg/kg
Nigella sativa
plus
highly active antiretroviral therapy respectively. The treatments
were given orally for 28 consecutive days. On the 29th day, all
rats were sacrificed under light diethyl ether anesthesia; blood
samples were collected for the assessment of biochemical
parameters, while liver tissue was used for histopathological
assessment.
Results:
Serum levels of liver enzymes ALT, AST, ALP, and GGT
were significantly (p<0.05) increasedandalbuminconcentration
was significantly decreased in animals treated with highly
active antiretroviral therapy as compared to the normal
control. Histopathological observations also revealed severe
damage in the structure of liver tissue in animals administered
with highly active antiretroviral therapy. Treatment of highly
active antiretroviral therapy exposed animals with
Nigella
sativa
showed marked improvement in both biochemical and
histopathological findings. Rise in liver enzymes was almost
restored to normal in animals treated with
Nigella sativa.
Conclusion:
Nigella sativa
, through its antioxidant activity,
effectively protects highly active antiretroviral therapy induced
liver toxicity.
Speaker Biography
Kissi Mudie has completed his MSc in Medical Biochemistry from Addis Ababa
University, School of Medicine. He is the Director of National Clinical Chemistry.
Laboratory, Ethiopian Public Health Institute. He has published more than 14 papers in
reputed journals and has been serving as an Associate Researcher
e:
kissimudiey@yahoo.com