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J Pharmacol Ther Res 2017 Volume 1 Issue 2

November 02-03, 2017 Chicago, USA

International Congress on

International Conference and Exhibition on

Drug Discovery, Designing and Development

Biochemistry, Molecular Biology: R&D

inding of cytochrome c (Cytc) to cardiolipin (CL) in the

inner mitochondrial membrane is involved with the onset

of apoptosis. In this study, we make use of CLcontaining

phospholipid monolayers to mimic the inner mitochondrial

membrane.Constant pressure insertion assay was employed

to monitor the Cytc-induced expansion of membrane

area. Simultaneous epifluorescence microscopy imaging

afforded the

in-situ

visualization of phospholipid demixing

and sorting in the membrane. The formation of a CL-rich Ld

phase has been observed to prelude the insertion of Cytc.

We will demonstrate that the insertion of Cytc disrupts the

membrane in a way facilitating the escape of CL. The findings

of our study may aid in understanding the early events

leading to the remodeling of inner mitochondrial membrane

and loss of its function during apoptosis.

Cytochrome c assisted escape of cardiolipin from a model mitochondrial membrane

Valeria Tsoukanova

and

Aby Thong

York University, Canada