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allied
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J Nutr Hum Health 2017 Volume 1 Issue 2
Notes:
July 24-26, 2017 | Vancouver, Canada
International conference on
DIABETES, NUTRITION, METABOLISM & MEDICARE
T
he aim of this study is preparation, optimization and
in
vitro
characterization of nanoparticles prepared from
octyl chitosan as a new strategy for oral insulin delivery. For
this study, N, N-dimethyl N-octyl chitosan was synthesized.
Nanoparticles containing insulin were prepared using PEC
method, and then were statistically optimized using the Box-
Behnken response surface methodology. The independent
factors were considered to be the insulin concentration,
polymer concentration and pH of the polymer solution,
while the dependent factors were characterized as the size,
zeta potential, PdI and entrapment efficiency. The optimized
nanoparticles were morphologically studied using SEM. The
cytotoxicity of the nanoparticles on the Caco-2 cell culture
was studied using the MTT cytotoxicity assay method, while
the permeation of the insulin nanoparticles across the
Caco-2 cell monolayer is also determined. Nanoparticles
posed appropriate physicochemical properties. The SEM
morphological studies showed spherical to sub-spherical
nanoparticles with no sign of aggregation. The
in vitro
release study showed that 95.5±1.40% of the loaded insulin
was released in 400 min. The cytotoxicity studies on the
Caco-2 cell culture showed no significant toxicity after 5 h
incubation. The permeability studies revealed significant
enhancement in the insulin permeability using nanoparticles
prepared from octyl chitosan at 240 min (11.3±0.78%). The
obtained data revealed that insulin nanoparticles prepared
from N, N-dimethyl, N-octyl chitosan can be considered as
the good candidate for oral delivery of insulin compared to
nanoparticles prepared from N, N, N- trimethyl chitosan.
Biography
Parham Norouzian is pharmacist student at Hamadan University of Medical Sciences.
He has published 2 articles in International journal of biology.
parham.norouzian@gmail.comNanoparticles prepared from N, N-dimethyl, N-octyl chitosan as the novel approach for oral delivery
of insulin: Preparation, statistical optimization and
in vitro
characterization
Parham Norouzian
and
Reza Mahjub
Hamadan University of Medical Sciences, Iran