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June 12-13, 2019 | Edinburgh, Scotland
Pediatrics and Clinical Pediatrics
2nd World Congress on
Current Pediatric Research | Volume: 23
Theranostic value of miR-499a seed region variant in Bronchial asthma
Eman A Toraih
Suez Canal University, Egypt
Background : Small non-coding RNAs (microRNAs) have
been evolved to master numerous cellular processes.
Genetic variants within microRNA seed region might
influence microRNA biogenesis and function. The study
aimed at determining the role of microRNA-499 (miR-499)
gene family polymorphism as a marker for susceptibility
and progression of bronchial asthma and to analyse the
structural and functional impact of rs3746444 within the
seed region.
Methods : Genotyping for 192 participants (96 patients
and 96 controls) in the discovery phase and 319 subjects
(115 patients and 204 controls) in the replication phase
was performed via Real Time-Polymerase Chain Reaction
technology. Patients underwent the methacholine
challenge test and biochemical analysis. Gene structural
and functional analysis, target prediction, annotation
clustering, and pathway enrichment analysis were
executed. Predicted functional effect of rs37464443 SNP
was analysed.
Results: miR-499 gene family is highly implicated in
inflammation-related signalling pathways. Rs374644 (A>G)
in MIR499A and MIR499B within the seed region could
disrupt target genes and create new genes. The G variant
was associated with high risk of developing asthma under
all genetic association models (G versus A: OR = 3.27, 95%
CI = 2.53-4.22; GG versus AA: OR = 9.52, 95% CI = 5.61-
16.5; AG versus AA: OR = 2.13, 95% CI = 1.24-3.46; GG + AG
versus AA: OR = 4.43, 95% CI = 2.88-6.82). GG genotype was
associated with poor pre-bronchodilator FEV1 (p=0.047)
and the worst bronchodilator response after Salbutamol
inhalation, represented in low peaked expiratory flow rate
(p = 0.035).
Conclusions : miR-499 rs3746444 (A>G) polymorphism was
associated with asthma susceptibility and bronchodilator
response in Egyptian children and adolescents. Further
functional analysis is warranted to develop more specific
theragnostic agents for selecting targeted therapy.
e:
Current Pediatric Research, Volume 23
ISSN: 0971-9032