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N o v e m b e r 1 2 - 1 3 , 2 0 1 8 | R o m e , I t a l y
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Journal of Chemical Technology and Applications
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Volume 2
J Chem Tech App 2018, Volume 2
FABRIC PHASE SORPTIVE EXTRACTION FOR THE DETERMINATION OF
LOCAL ANESTHETICS FROM BIOLOGICAL SAMPLES
Ripneel Kaur
Punjabi University, India
A
novel, simple and sensitive high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been
developed and validated for simultaneous quantification of three local anesthetics namely lidocaine, prilocaine and dibu-
caine in blood serum and urine. New sorptive technique has been used for preconcentration of anesthetics in the various sam-
ples of blood serum and urine followed by the HPLC-UV detection. Various factors which can influence the performance of fiber
phase extraction like extraction time, back extraction time, eluting solvent and volume of extracting solvent etc were optimized.
The chromatographic separation was achieved using a simple mobile phase consisting of acetonitrile: water (70:30 v/v) at an
isocratic flow of 0.5mL/min using HPLC (Dionex softron GmbH, Germany) setup consisted of a P 680 quaternary solvent delivery
pump, a column o C18 column (100 × 4.6 mm, i.d., 5 μm) and UV detector. The separation was performed on wavelength of
230nm. The calibration curves of target analytes were prepared in the concentration range 5–500 ng/mL with good coefficient of
determination values (R2 >0.992).. The limits of detection range from 0.050 to 0.160µg/mL.