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Journal of Biotechnology and Phytochemistry
Volume 1 Issue 3
Chemistry World 2017
Notes:
Page 28
November 13-15, 2017 Athens, Greece
7
th
World Congress on
Chemistry
Comparison of Different Sample Preparation
Techniques for the Analysis of Small Molecules in
Biological Fluids
Sena Caglar Andac
Istanbul University, Turkey
B
iological matrices consisting of high molecular weight
matrices i.e. endogenous substances, metabolites, proteins,
blood cells and coexisting drugs are often difficult to handle
for chromatographic analysis. To purify and enrich the target
analytes and drugs in these complex matrices an effective sample
pretreatment step is essential. Biological sample pretreatment
has always been a forgotten part of the biopharmaceutical
analysis. Among the sample pretreatment techniques, traditional
off-line sample processing like liquid-liquid extraction, protein
precipitation, and solid phase extraction are gradually becoming
a limiting bottleneck in the chromatographic analysis. On-line
and fully automated techniques have been in demand recently.
For this purpose, new systems and coupled devices put on the
market by several companies. In this presentation, importance of
sample pretreatment in chromatography, manual and automated
sample preparation techniques, comparison of these techniques
and chromatographic method development steps will be
discussed. Some applications of drug determination in complex
bio-fluids will be presented under the consideration of current
publications. Current trends and the future prospects of sample
preparation will be overviewed.
Biography
Sena Caglar Andac has completed her PhD from Istanbul University and
Postdoctoral studies from Medical Center of Munich University, Laboratory
of Bio-Separation. Currently, she is working as an Associate Professor at
Istanbul University, Faculty of Pharmacy. She has researches published in
liquid chromatography mass spectrometry for biological fluid analysis of drugs,
on-line solid phase extraction coupled liquid chromatography, determination
of drugs and degradation studies by high performance liquid chromatography,
spectrophotometry and spectrofluorimetry.
sena@istanbul.edu.trSena Caglar Andac, J Biotech and Phyto 2017