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Page 14
J Parasit Dis Diagn Ther 2017 | Volume 2 Issue 4
allied
academies
International Conference on
Zoology, Microbiology & Medical Parasitology
October 30-November 01, 2017 | Chicago, USA
Notes:
T
he methods currently used in FDA field laboratories and
other public health laboratories for detecting
Salmonella
in
food /environmental samples require 2 days and have limited
sensitivity. We describe the development and validation of a
real-time PCR method that detected
Salmonella
and presence
of group D in 24 h. Primers and probes specific to the
invA
gene of
Salmonella
, group D, and Enteritidis serovar were
designed and evaluated for the inclusivity and exclusivity using
a panel of 329
Salmonella
isolates consisting 126 serovars
from 32- O groups and 22 non-
Salmonella
environmental
organisms. The invA-, group D-and Enteritidis - specific sets
identified the isolates accurately. The PCR method was100%
inclusive for
Salmonella spp
and had a detection limit of 2 copies
of
Salmonella
DNA per reaction. A Single-laboratory validation
performed on 1,741 environmental samples demonstrated that
the PCR method detected 55% more positives than the VIDAS
method that is currently used. The method is more specific
and did not report any false-negatives. The receiver operating
characteristic (ROC) analysis documented excellent agreement
between the results from the culture and PCR methods (area
under the curve, 0.90; 95% confidence interval of 0.76 to 1.0)
confirming the validity of the PCR method. The validated PCR
method will help to strengthen public health efforts through
rapid screening of
Salmonella spp
. in environmental samples.
Speaker Biography
Kuppuswamy N Kasturi after completing DSc from the University of Paris South, France,
pursued postdoctal studies at the Beatson Institute for Cancer Resaerch, Glasgow,
Scotland, United Kingdom and then worked as a Member of Microbiology Faculty at
Mount Sinai Meical Center, New York, USA prior to joining USFDA as a Microbiologt in
2002. He has published more than 50 papers in reputed international journals and has
been serving as an Editorial Board Member of
International Journal of Food Science,
Nutrition and Dietetics
.
e:
Kuppuswamy.Kasturi@fda.hhs.govKuppuswamy N Kasturi
US Food and Drug Administration, USA
Real-Time PCR for detection of Salmonella
spp.
in environmental samples