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allied
academies
Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
D
ue to increased oil demand, depleting fossil fuels and
greenhouse gas emissions, biofuels production are
getting much attention. The fatty acid based biofuels (fatty
acids ethyl ester/biodiesel, fatty alcohol, etc.) produced from
microbial cells have emerged as ideal alternatives to fossil
oils, with significant pluses over plant, animal and algae oils.
Saccharomyces cerevisiae
is a most studied industrial model
microorganism and also its fatty acid production ability has
been increased by metabolic engineering approach. But
still the cost of the process limits its industrial production
therefore, more research is required. Here, we are addressing
this issue by sequential metabolic engineering approach. In
order to synthesize biodiesel in yeast cells, we integrated
wax ester synthase (WS2) gene from
Marinobacter
hydrocarbonoclasticus
into its genome. The genetic
engineering approaches have focused on high-level biodiesel
production by rewiring metabolism pathways to upsurge
carbon flux towards fatty acid CoA synthesis, by increasing
the cofactor supply, and disrupting the degradation pathway.
e:
priya7654@gmail.comBioengineering of yeast cell for biodiesel production
Priya Kumari
and
Naseem A Gaur
DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi