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World Biochem 2019 & Regenerative Medicine 2019
Journal of Genetics and Molecular Biology | Volume 3
Page 54
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
March 25-26, 2019 | Amsterdam, Netherlands
&
BIOCHEMISTRY AND ENZYMOLOGY
World Congress on
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH
2
nd
Global Conference on
Joint Event on
THE ROLE OF THE FLOW RATE ON CELL DIFFERENTIATION DURING
SEEDING OF THE STEM CELLS WITHIN THE MATRIX
Zahra Eslami
Shahid Beheshti University of Medical Sciences, Iran
C
ardiovascular disease (involving narrowed or blocked blood vessels which can lead to heart attack or
stroke) is a widespread disease throughout the world and conventional surgical implantation procedures
such as coronary artery or peripheral by-pass require autologous vessels or synthetic grafts with diameters
lower than 5 mm. In this case, tissue-engineered vascular grafts are considered as an appropriate alternative
which are typically fabricated by seeding the stem cells into a porous tubular scaffold. However, the method
by which the stem cells are seeded within a 3D tubular scaffold can be a dramatically decisive parameter to
achieve a fully functional vascular graft. Spatially-uniform cellular distribution throughout the thickness of the
tubular tissue-engineered graft is particularly of great importance since it provides the required conditions for
uniform regeneration of the tissue. Also, the stem cell viability is often challenging because the mechanical
driving forces used for seeding step can cause shear-mediated membrane lysis or may result in triggering of
apoptotic pathways. To address these problems, simultaneous application of centrifugal force and the pump-
ing flow offers a promising seeding procedure. As a particular item, various flow rates during incorporation of
the stem cells within tubular scaffolds play a key role on cell differentiation in terms of cell number, distribu-
tion, viability and phenotype. The change in the applied volume of the flow as well as the change in the corre-
sponding frequency leads to alterations in shear stress on protoplasm of the stem cells, which in turn results
in differentiation of these cells to myocytes. The mentioned changes in the volume and frequency of the flow
are applied by changing the diameter of the lumen.
J Genet Mol Biol 2019, Volume 3