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World Biochem 2019 & Regenerative Medicine 2019
Journal of Genetics and Molecular Biology | Volume 3
Page 53
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
March 25-26, 2019 | Amsterdam, Netherlands
&
BIOCHEMISTRY AND ENZYMOLOGY
World Congress on
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH
2
nd
Global Conference on
Joint Event on
CONSTRUCTION OF DISCRETE MODEL OF HUMAN PLURIPOTENCY
IN PREDICTING LINEAGE-SPECIFIC OUTCOMES AND TARGETED
KNOCKDOWNS OF ESSENTIAL GENES
Priyanka Narad
Amity University, India
A
network consisting of 45 core genes was developed for the genes/proteins responsible for loss/gain of
function in human pluripotent stem cells. The nodes were included on the basis of literature curation.
The initial network topology was further refined by constructing an inferred Boolean model from timeseries
RNA-seq expression data. The final Boolean network was obtained by integration of the initial topology and
the inferred topology into a refined model termed as the integrated model. Expression levels were observed
to be bi-modular for most of the genes involved in the mechanism of human pluripotency. Thus, single and
combinatorial perturbations/knockdowns were executed using an insilico approach. The model perturbations
were validated with literature studies. A number of outcomes are predicted using the knockdowns of the core
pluripotency circuit and we are able to establish the minimum requirement for maintenance of pluripoten-
cy in human. The network model is able to predict lineage-specific outcomes and targeted knockdowns of
essential genes involved in human pluripotency which are challenging to perform due to ethical constraints
surrounding human embryonic stem cells.
J Genet Mol Biol 2019, Volume 3