8 / 13
Page 44
allied
academies
Virol Res J 2017 Volume 1 Issue 3
International Virology Conference
October 30-31, 2017 | Toronto, Canada
C
apripoxviruses are genetically and antigenically similar.
Sheeppox virus (SPPV) and goatpox virus (GPV) cause
diseases in ovines and caprines, respectively. Lumpy skin
disease virus (LSDV) causes lumpy skin disease (LSD) in cattle.
LSD is endemic in Africa and theMiddle East, andwas recently
introduced into Europe and Russia. Live attenuated SPPV is
used as a vaccine in endemic areas. Cattle vaccinated using
SPPV can develop LSD due to induction of partial protection,
or as a result of vaccine seed contamination with non-highly-
attenuated LSDV. LSD control and vaccine production can be
enhanced by differentiation between LSDV and SPPV using
a highly specific, simple, rapid, and inexpensive PCR assay.
In this study, primers were designed to specifically amplify
conserved LSDV sequences spanning parts of LSDV100, and
LSDV101 genes. The design allowed the amplification of a 503
bp PCR product that was used for diagnosis. An alternative
reverse primer allowed the amplification of a LSDV-specific
1583 bp PCR product for sequencing. The diagnostic assay
detection limit was 585 genome-copy-equivalents of LSDV/5
ul of extract. A real-time assay was 10 times more sensitive.
LSDV DNA was detected in skin samples collected from 1988
to 2015. Amplification of LSDV sequences was not affected
by lesion size and distribution (localized or generalized)
on infected animals. Application of the developed assay
for the quality control of local LSD vaccines resulted in the
detection of LSDV contamination of a local SPPV vaccine.
The incorporation of the developed assay in LSD control
programs was recommended.
e:
ausama_yousif@cu.edu.egLSDV100 and LSDV101 lumpy skin disease virus-specific PCR and real-time PCR for rapid diagnosis and
vaccine quality control
Ausama A Yousif
Cairo University, Egypt