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allied
academies
Virol Res J 2017 Volume 1 Issue 3
International Virology Conference
October 30-31, 2017 | Toronto, Canada
T
he worldwide importance of rice, Oryza
sativa
, as food
crop is well-understood. In order to maintain a consistent
supply of rice globally, control of pathogens affecting crop
production is matter of due concern. Rice yellow mottle
virus (RYMV) is known to cause a variety of symptoms in
Oryza
sativa
plants and certain symptoms account for death
and hence reduce yield. Four ORFs can be identified in
the genome of RYMV encoding for P1 (ORF1), Polyprotein
(processed to produce VPg, protease, helicase, RdRp4);
(ORF2), putative RdRp; (ORF3) and capsid/coat protein
(ORF4). This research was executed to identify genome
encoded miRNAs of
O.
sativa
that are targeted to the
genome of Rice Yellow Mottle Virus (RYMV). A consensus of
four prediction algorithms (RNA22, miRanda, TargetFinder
and psRNATarget was considered, thus allowing a multitude
of miRNA target prediction parameters to be implemented
including minimum free energy of binding, folding energy,
seed pairing, target site accessibility and multiple target
sites. A phylogenetic tree was constructed to portray the
evolutionary relationships between RYMV strains isolated
to date. Finally, target site conservation was also evaluated
which revealed a varying degree of miRNA target site
conservation in the genome of RYMV. Results of this research
are expected to act as precursor for the development of
RYMV resistant rice varieties around the world by using
recombinant expression of selected miRNAs in
O. sativa
.
e:
jean-pierre.perreault@usherbrooke.caIdentification of miRNA for expression in rice to provide resistance to RYMV
Muhammad Shahzad Iqbal
1
, Basit Jabbar
1
, Idrees A Nasir
1
and
Robert J Henry
2
1
University of the Punjab, Pakistan
2
QAAFI- The University of Queensland, Australia