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alliedacademies
Virology Research Journal
Volume 1 Issue 4 Vaccines World 2017
Notes:
Page 38
November 09-10, 2017 Vienna, Austria
21st World Congress and Exhibition on
VACCINES, VACCINATION & IMMUNIZATION
Downstream processing of gene therapy vectors and
vaccines using monolithic columns
Ingo Nagler
1BIA Separations, Slovenia
2Vienna University of Technology, Austria
3Exputec GmbH, Austria
Production of high value biological therapeutics usually
involves complex manufacturing processes with high
process variability. Additionally, development of robust and
reliable bioprocesses can be challenging. PAT aims to enhance
bioprocess understanding and implies a holistic approach to
ensure that quality is built into products by design. Efficient PAT
therefore calls for fast and robust analytical techniques which
enables to asses high quality information about critical quality
attributes and key performance indicators as parallel as possible
to the manufacturing process. PATfix™ is unique HPLC system
for routine gradient separations that enables every analytical
task. Equipped with bio-inert ceramic pump heads is deliberately
tailored to meet the demands of analytical applications covering
wide range of biomolecules. Highly sensitive and fast multi-
wavelength detector enables to detect component peaks even in
very fast gradients.
Recent Publications
• E LIMONTA, Miladys, et al. "Comparison of CIM® C4⁃
HLD monolithic column with Sartobind phenyl membrane
column for pIDKE2 purification." Chinese Journal of
Chromatography 1028: 1036.
• Vincent, David, et al. "The development of a monolith-based
purification process for Orthopoxvirus vaccinia virus Lister
strain." Journal of Chromatography A (2017).
• Lesch, H. P., et al. "Production and purification of lentiviral
vectors generated in 293T suspension cells with baculoviral
vectors." Gene therapy 18.6 (2011): 531-539.
Biography
BIA Separations is the leading developer and manufacturer of Convective
Interaction Media (CIM) monolithic columns for fast purification of biomolecules.
Our goal is to provide the best solutions in downstream processing and
analytics, with the goal of saving our customers valued time and costs during
the production of their desired biomolecules. We have over 20 years’ experience
in chromatographic purification of large biomolecules including viruses, VLPs,
phages, pDNA, antibodies (IgM, IgG), proteins, as well as removal of viral
particles, endotoxins and proteins from different biological matrices. We provide
products that range from small analytical columns to large industrial prep-
scale columns (including cGMP and disposable units in IEX, HIC, affinity and
customized formats) as well as method development services.
Ingo Nagler, Virol Res J 2017, 1:4