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Journal of Clinical and Experimental Toxicology | Volume: 2
December 03-04, 2018 | Dubai, UAE
International Conference on
6th International Conference on
Toxicology, Clinical Toxicology & Pharmacology
Recycling & Waste Management
Joint Event
&
Garlic as an antimalarial drug in Artemisinin Combination Therapy
Vathsala P G
Indian Institute of Science, India
Garlic (Allium sativum) is one of the popular herbal
medicines used worldwide to reduce various risk factors
associated with several diseases. Garlic contains a variety of
effective compounds that exhibit anticoagulant, antioxidant,
antibiotic, hypocholesterolaemic and hypoglycaemic as well as
hypotensive activities. To evaluate antimalarial activity of garlic
pearl oil and artemisinin in combination therapy, Commercially
available α-β Arteether (E MALTM) and Garlic pearl oil
(Ranbaxy Laboratories Ltd., New Delhi, India) were tested for
its antimalarial activity in Plasmodium berghei-infected mouse
model. This study demonstrates, for the first time, the in vivo
antimalarial activity of arteether and garlic pearl either as
individual molecules or in combination at various dosage levels
in Plasmodium berghei-infected mouse model of malaria. After
72 h (Day 3) when the parasitemia was about 2-4%, infected
mice were treated with single dose intramuscular injection of
750 μg of arteether in combination with three 100 μL oral
doses of garlic pearl on Day 3, Day 4 and Day 5 and showed
100%protection against malaria. Giemsa stained blood pictures
show inhibition of parasitemia in combination drug treated
animals and the protection during recrudescence interval in
arteether monotherapy. This approach shows that arteether
and garlic pearl oil combination therapy gives complete
protection in P. berghei-infected mice. Arteether and garlic
combination therapy resulted in a striking increase in anti-
parasite antibody IgG contributing protective immunity during
the recrudescence phase. These results are also matching with
Western Blot analysis, where sera from the recrudescence
stage and later period of AE+garlic treated animals interacted
with several parasite specific proteins, compared to the
controls. There is potential to decrease the dose of artemisinin
and in developing low-cost antimalarial drug therapies and
for the first time garlic appears to be an ideal antimalarial
molecule especially for use in artemisinin combination therapy.
Speaker Biography
Vathsala P G has been working on combination therapy for malaria for more than two
decades and completed her PhD from Indian Institute of Science. She is currently serving
in Biology Division of Undergraduate Programme along with research activity. She has
published 10 papers in reputed journals on antimalarial drugs
e:
Vathsala P G, Toxicology 2018 & Recycling 2018, Volume 2
DOI: 10.4066/2630-4570-C1-002