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May 20-21, 2019 | Rome, Italy
Journal of Cell Science and Mutation | Volume 3
OF EXCELLENCE
IN INTERNATIONAL
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Stem Cell Congress 2019
STEM CELLS AND REGENERATIVE MEDICINE
2
nd
International Conference on
HOFFA’S FAT-KEY TO UNLOCK CARTILAGE REGENERATION
Radhesh R Menon
1
, Siddharth Shetty
1
, Vikram Shetty
1
, B Mohana Kumar
2
and
Narendra N
2
1
Department of Orthopaedic Surgery, K S Hegde Hospital, India
2
NITTE University Centre for Stem Cell Research and Regenerative Medicine, K S Hegde Medical Academy, India
O
steoarthritis is the most common progressive degenerative disease affecting the knee joint. Osteoarthritis
represents a disease where the prospects of stem cell based therapy offers considerable hope. Hoffa’s fat
aka infra patellar fat pad (IPFP) an intra-articular and extra synovial structure in the knee joint are capable of
chondrogenic differentiation and this property can be exploited to address the damage to the articular carti-
lage. Stem cells obtained from IPFP were analyzed based on various cellular characteristics, such as the yield,
viability, growth kinetics, colony-forming ability and expression of selected phenotypic markers. The fat pad
samples with prior consents were obtained from patients who presented for primary total knee arthroplasty
to the Department of Orthopedic Surgery at K S Hegde Medical Academy. The fat pad samples were processed
at the Stem Cell Laboratory at KSHEMA. A total of five samples were used for the establishment of stem cell
lines. Nucleated cell number per one gm fat tissue ranged from 0.36 million to 1.05 million and total adherent
cell number after 14 days culture per one gm fat tissue was varied from 0.3 million to 1.0 million, showing the
potential of high yield. Isolated cells exhibited small spindle-shape morphology and the viability was >97.5% at
all passages examined (P1-P3). Proliferation rate of IPFP-stem cells was high and average population doubling
time was found to be 66.32±6.60 hrs. As a potency marker, IPFP-stem cells showed high colony formation abil-
ity. Flow cytometry analysis revealed that cell populations were over 70% positive for the mesenchymal stem
cell (MSC) markers, such as CD29, CD44, CD73 and CD90, but CD146 and CD166 showed only <10% positivity.
In addition, IPFP stem cells are also being assessed for their chondrogenic potential. To conclude, IPFP derived
stem cells have rich yield and viability and were shown to be highly proliferative with expression of phenotypic
markers specific to multipotent stem cells.
Radhesh R Menon et al., Adv Cell Sci Mut. 2019, Volume 3
Radhesh R Menon is a final year post graduate student pursuing Orthopaedic Surgery at K S Hegde Hospital which is a part of the
well-known NITTE University, India.
radhesh.menonr@gmail.comBIOGRAPHY