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Virology Research Journal | Volume 2
allied
academies
May 18-19, 2018 | Montreal, Canada
2
nd
World Conference on
STDs, STIs & HIV/AIDS
Background:
The HIV 1 integrase catalyses the chromosomal
integration of newly synthesized double stranded RNA into
the host genomic DNA. Some countries have incorporated
integrase inhibitors as components of antiretroviral drug in
curtailing the replicative proclivity of the AIDS virus. The aim
of this study was to analyse the complete HIV 1 integrase
gene obtained from newly diagnosed HIV 1 pre-HAART
patients from selected clinics in the Eastern Cape South
Africa.
Methods:
Sequencing of the HIV 1 integrase region from
plasma samples of 45 newly diagnosed drug naive patients
were carried out using ABI PRISM genetic analyser and
edited with the Geneious 10.3 software while complete
sequenced results were obtained from the HIV sequences in
32 patients. Drug resistance mutation to the integrase was
inferred from the HIV 1 Stanford drug resistance database
while phylogenetic analysis was done to classify the viral
sequences into subtypes.
Results:
No major resistance to the integrase sequence was
observed. Only 1(3.1) case of polymorphic accessory E157Q
mutation was observed. Another minor mutation (L74M/L)
which is a highly polymorphic accessory mutation was
identified in 1(3.1%). Phylogenetic analyses classified all the
sequences as HIV 1 subtype C.
Conclusion:
The present study indicates that no major
integrase mutation was observed and the data obtained
will contribute to the relevance of integrase polymorphism
and improve resistance interpretation algorithm among
the subtype C. Apparently more sample size and additional
studies may be essential to evaluate the impact of these
mutations on integrase inhibitors prior to their introduction
into South Africa in the nearest future.
e:
digbant@gmail.comMolecular analysis of HIV-1 integrase sequences among Pre-HAART patients in the Eastern Cape,
South Africa
Digban T
University of Fort Hare, South Africa