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Materials Science and Nanotechnology | Volume: 03
WORLD CONGRESS ON SMART MATERIALS AND STRUCTURES
&
3
rd
International Conference on
POLYMER CHEMISTRY AND MATERIALS ENGINEERING
November 21-22, 2019 | Singapore
Joint event on
I
n joint arthroplasty, Ultra High Molecular Weight
Polyethylene (UHMWPE) is used as a bearing surface due
to its excellent attributes, such as good wear resistance,
high strength, bio compatibility, lightweight, chemical
stability, and lubricity. We have shown in previous works,
the ability of UHMWPE coatedwith nylon in enhancing both
mechanical and biological performances of conventional
UHMWPE, including superior tensile strength, higher
impact resistance, reduced damage, and improved cell
viability. The purpose of the current study is to assess the
wound healing and the antimicrobial capabilities of this
novel nylon-coated UHMWPE. A combination of biological/
biochemical tests, including MTT viability, antibacterial
activity (using E. coli and S. aureus), and wound healing
assays were performed to assess the bio activity and the
bio compatibility of the coated specimens. Additional tests,
such as SBF absorption, alizarin staining, SEM, EDX, and
FTIR techniques were conducted to evaluate the moisture
uptake, osteogenic activity, and the morphology of the
coated samples. Our results indicate that the viability of
U2OS (osteosarcoma cell line) cells cultured in conditioned
media for 48 hours was comparable for both coated (95%)
and uncoated (93%) UHMWPE relative to the control.
Interestingly, the antibacterial activity test results after 24
hours incubation exhibited that the nylon coated UHMWPE
has significantly higher antibacterial activity compared to
the uncoated UHMWPE. There was no bone mineralization
detected in U2OS as no Alizarin red stain was observed,
confirming the absence of any osteogenic induction. Such
quality is a preferred characteristic of bearing surfaces.
In agreement with Alizarin red staining results, the EDX
demonstrated the absence of a hydroxyapatite layer, which
is a required feature in the bearing surfaces. The uptake
of the simulated body fluid (SBF) by both the coated and
uncoated UHMWPE indicated that the nylon coating is of
great advantage to the polymer as it prevents the increased
absorption of the SBF and hence may decrease the risk of
implant degradation. Moreover, our results demonstrate
the superiority of the proposed coated biomaterial for
wound healing applications with improved antibacterial
capabilities.
Biography
Asma Amleh is an Associate Professor of Biology at The American
University in Cairo and an Adjunct Professor, Department of Mechanical
& Industrial Engineering at Ryerson University, Toronto, Canada. She
received a BSc in biology and chemistry at The American University
of Beirut, Lebanon, and a Ph.D. in biology from McGill University in
Montreal, Canada (1997). She has been a research fellow at the laboratory
of cellular and developmental biology, NIDDK, and a research associate
at the National Institutes of Health, Bethesda, MD, USA. She has also
been an instructor at the Department of Molecular Medicine, Institute
of Biotechnology, at the University of Texas Health Science Center and a
senior research scientist at the Developmental Biology Program, Memorial
Sloan-Kettering Cancer Center in NY. Her research interests are focused on
understanding the genetic control of normal and abnormal development
in the mammalian system, including tissue compatibility of biomaterials
and the incidence of cancer.
e:
aamleh@aucegypt.eduAsma Amleh
The American University in Cairo, Egypt
Nylon-coating improves the performance of UHMWPE bearing surfaces
Mater Sci Nanotechnol, Volume: 03