probiotics-pediatrics-congress-2019-accepted-abstracts

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Journal of Public Health and Nutrition | Volume 2

July 05-06, 2019 | Paris, France

International Conference and Exhibition on

World Congress on

Probiotics, Nutrition and Functional Foods

Pediatrics and Nutrition

Joint Event

Identification of potential probiotic candidates to prevent oral candidiasis

Juliana Campos Junqueira, Rodnei Dennis Rossoni, Patrícia Pimentel de Barros1, Felipe de Camargo Ribeiro,

Beth Burgwyn Fuchs

and

Eleftherios Mylonakis

Institute of Science and Technology, São Paulo State University/UNESP, Brazil

Rhode Island Hospital, Alpert Medical School of Brown University, USA

tudies focused on antifungal activity of Lactobacillus may

contribute to the discovery of new therapeutic strategies

for Candida infections. In the previous studies of our research

group, we isolated and identified Lactobacillus spp. from

the oral cavity of caries-free subjects to seek for strains with

antifungal activity against Candida albicans. Firstly, the effects

of 30 clinical isolates of Lactobacillus were evaluated on the

number of viable cells of C. albicans in biofilms and on hyphae

formation by in vitro assays. The results demonstrated that

L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4

were the strains with the highest antimicrobial activity on C.

albicans. These strains were able to reduce the biofilms by

decreasing the total biomass, changing the morphological

architecture and downregulating the gene expression of C.

albicans (ALS3, HWP1, EFG1 and CPH1). In the in vivo study,

the injection of L. paracasei 28.4 into the Galleria mellonella

increased the survival rate, the number of hemocytes and the

expression of antifungal peptides, thus reducing the CFU of

C. albicans. In Caenorhabditis elegans, L. paracasei 28.4 was

also able to increase the survival of worms infected with C.

albicans and reduce the filamentation. We conclude that L.

fermentum 20.4, L. paracasei 28.4 and L. rhamnosus 5.2 have

potential to be used as probiotics in the oral cavity to control

Candida infections.