probiotics-pediatrics-congress-2019-accepted-abstracts

allied

academies

Page 45

Journal of Public Health and Nutrition | Volume 2

July 05-06, 2019 | Paris, France

International Conference and Exhibition on

World Congress on

Probiotics, Nutrition and Functional Foods

Pediatrics and Nutrition

Joint Event

Two-dimensional cultured intestinal stem-cell derived organoids as a model to test

nutritional compounds for intestinal health

Laura M’Rabet, Stefan Vaessen, Jean Paul Ten Klooster, Saertje Verkoeijen, Annemarie Stam, Nikkie van der

Wielen, Jocelijn Meijerink, Renger Witkamp, Cyrille Krul

and

Raymond Pieters

University of Applied Sciences, The Netherlands

e developed a two-dimensional (2D) intestinal

stem cell-derived organoid model with both crypt

and differentiated villus cells (enterocytes, goblet cells

and enteroendocrine cells) to study safety and efficacy of

compounds. Organoids were grown from duodenal, jejunal

and ileal tissue. We show that these 2D organoid cultures

maintain location-specific gene expression and responses,

e.g. that the artificial sweetener rebaudioside A (derived

from Stevia) stimulates production of GLP1 specifically

by ileal enteroendocrine cells. Furthermore, we grow 2D

intestinal organoid cultures in transwell format to confluent

monolayers with build-up of electrical resistance, low FD4

leakage and responsiveness to cytokines. Our data indicates

that our 2D intestinal organoid model allows studying effects

of compounds beyond possibilities with standard epithelial

cell lines. We are currently exploring this culture method to

investigate effects of other nutritional compounds on the

intestinal uptake and mucin production.