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November 13-14, 2017 Paris, France
5th International Conference on
PLASMA CHEMISTRY AND
PLASMA PROCESSING
Journal of Biotechnology and Phytochemistry
Volume 1, Issue 2 Plasma Chemistry 2017
Head and neck cancer treatment with plasma
activated medium
Merbahi N1, J Chauvin1, 2, M Yousfi1 and P Vicendo2
1LAPLACE - University of Toulouse, France
2IMRCP - University of Toulouse, France
Biomedical applications of low-temperature plasmas are
of growing interest. These plasmas jet are an interesting
source of active species (charged particles, radicals, long-lived
excited species, UV photons, electric field, etc.) that can easily
be launched on any prokaryote or eukaryote cells, living tissues,
biomaterial surfaces. The present work is aimed at investigating
the regionalized antiproliferative effects plasma activated
medium (PAM) on multicellular tumor spheroid (MCTS), a
model that mimics the 3D organization and the regionalization
of a microtumor region. A homemade helium plasma jet
was used to produce PAM. In the case of multicellular tumor
spheroids, results indicate that PAM can induce cell detachment
in the first day in a PAM time-dependent manner associated
with the regionalized accumulation of DNA damage detected
by histone H2AX phosphorylation. This DNA damage is due to
the presence of hydrogen peroxide in PAM. However, a cellular
protective response that defends FaDu cells against H2O2 is
observed and a rapid spheroids regrowth is occurring. After
multiple PAM treatments of FaDu, MCTS growth inhibition
is obtained. Finally, this study underlines the importance of
working with MCTS instead of 2D cells. Indeed, after PAM
treatment, monolayer culture using a high number of cells has a
response at day one close to the MCTS one. But in the following
days, cells behaviors diverge. Contrary to MCTS that have a high
proliferation rate at day two, cells in 2D culture continue to die.
Observations on 2D cell culture can suggest that a single PAM
treatment is enough to kill cancerous cells. Our results clearly
demonstrate that MCTS models, closer to an In vivo tumor,
displayed a defense response leading to a growth increase of
spheroids which requires adaptation of treatment with PAM.
Biography
Merbahi N has completed his PhD from Paul Sabatier University, France. He is
the professor in Toulouse University, France. He has over 60 publications that
have been cited over 300 times, and his publication H-index is 15.
Merbahi N et al., J Biot Phyt 2017