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N o v e m b e r 2 3 - 2 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
Plant Science Congress 2018
Journal of Agricultural Science and Botany
ISSN: 2591-7897 | Volume 2
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
PLANT GENOMICS
AND PLANT SCIENCE
World Congress on
Uma Priya Kupusamy, J Agric Sci Bot 2018, Volume 2 | DOI: 10.4066/2591-7897-C2-005
IDENTIFICATION OF TRANSCRIPTION
FACTORS INVOLVED IN THE RESPONSE TO
BOTH BPH INFESTATION (BIOTIC STRESS)
AND DIFFERENT LEVELS OF NITROGEN
(ABIOTIC STRESS) IN RICE CULTIVARS
Uma Priya Kupusamy
Newcastle University, United Kingdom
P
lants have evolved to develop astonishing survival strategies to adapt to
variations in environmental conditions include rapid onset of abiotic and
biotic stresses. These extreme conditions have caused constraints on the
growth and development of plants as well as caused enormous economic
loss globally to crops. Rice,
Oryza sativa
is one of the most important staple
foods for more than half of the world population. In order to fulfil the food de-
mand of the growing population, rice production needs to be increased signifi-
cantly to 42%, from its current level. Stresses such as nitrogen (N) deficiency
(abiotic stress) and brown planthopper (BPH) infestation (biotic stress) has
been a major constraint in rice growing areas. The present study identified
two TF genes which were involved in the combination of the reduced levels
of N and BPH infestation in TN1 (susceptible to BPH) and IR70 (resistant to
BPH) rice cultivars.
OsNCL1
and
OsNCL2
which was previously reported to
be potentially related to BPH-resistance showed differential expression pat-
terns in response to the combination of both the stresses. These TFs were
up-regulated in response to the reduced levels of N (1.04 mM NH
4
NO
3
, 0.64
mM NH
4
NO
3
and 0.24 mM NH
4
NO
3
) compared to the optimal N level (1.44
mM NH
4
NO
3
) at different time points of BPH infestation whilst the resistant
IR70 was down-regulated to a greater magnitude in response to the reduced
levels of N compared to the optimal upon BPH infestation. Down-regulation
of both the genes in the resistant IR70 cultivar under the reduced levels of N
and in the presence of BPH infestation shows that these TFs have repressed
many active pathways to prevent further damage and is an efficient method
of defence against infestation of the insect pest. Ultimately understanding
the gene-regulatory network is important to develop or select for stress-toler-
ant and high yielding rice cultivars.
Uma Priya Kupusamy has completed her PhD recently
(Jun 2018) from Newcastle University, United Kingdom.
Her field of study involved molecular responses of rice
to abiotic and biotic stress. She is currently the head of
food microbiology section in the department chemistry,
Malaysia and also a member of reputed working groups
in Malaysia.
uma.priya0406@gmail.comBIOGRAPHY