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N o v e m b e r 2 3 - 2 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
Plant Science Congress 2018
Journal of Agricultural Science and Botany
ISSN: 2591-7897 | Volume 2
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
PLANT GENOMICS
AND PLANT SCIENCE
World Congress on
Puzon Juliana Janet M, J Agric Sci Bot 2018, Volume 2 | DOI: 10.4066/2591-7897-C2-005
OPTIMIZING THE SEED GERMINATION OF
GARCINIA MANGOSTANA
L. THROUGH
PRIMING TREATMENTS
Puzon Juliana Janet M
University of the Philippines, Philippines
G
arcinia mangostana
L., commonly known as mangosteen, has recalcitrant
seeds that remain dormant unless exposed to optimal environmental con-
ditions. In various crops, seed priming treatments are known to enhance seed
germination, and alter the seed’s metabolic activity before germination. The
study aimed to optimize the germination of
G. mangostana
seeds through
different priming treatments, namely, soaking the seeds in H
2
O, H
2
O
2
and acid
scarification with HNO
3
. The effectiveness of the priming treatments in induc-
ing seed germination was compared. The lipid and carbohydrate components
of the seeds that had undergone primingwere determined. Mangosteen seeds
were subjected to three different methods of seed priming, namely soaking
seeds in distilled water, H
2
O
2
acid scarification with HNO
3
before germinating
in the dark for seven days. The germination percentage, mean germination
time, and germination rate were measured every day. After 7 days of germi-
nation, total lipids and total carbohydrates in ungerminated and germinated
seeds were quantified using modified Folch method, and Dubois assay, re-
spectively. The results of seed germination showed that soaking the seeds in
0.5% H
2
O
2
was the most favourable among all the priming treatments based
on the values of measured germination parameters. A significant difference
between the mean % germination of seeds was observed, while there was no
significant difference between the mean germination rates and mean germi-
nation time of seeds between treatments. Increasing concentrations of H
2
O
2
and HNO
3
reduced the lipid content of seeds. The carbohydrate content of
the germinated seeds primed with distilled water and increasing concentra-
tions of H
2
O
2
decreased, while seeds primed with increasing concentrations
of HNO
3
had increasing carbohydrate content. Therefore, the concentrations
of H
2
O
2
and HNO
3
in the priming treatments greatly affect the lipid and car-
bohydrate contents of the seeds. Results of this study serve as a significant
contribution to the improved propagation of
G. mangostana
L., an economi-
cally valuable species.
Puzon Juliana Janet M is a botany professor in the In-
stitute of Biology, College of Science, University of the
Philippines, Diliman, Quezon City. She teaches botany
subjects and heads the Plant Physiology Research Lab-
oratory in this institute. Her current research interests
include plant stress physiology, phytotechnologies, bio-
active secondary metabolites, and physico-chemical and
phytohormonal control of seed germination.
janetmpuzon@gmail.comBIOGRAPHY