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N o v e m b e r 2 3 - 2 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
Plant Science Congress 2018 Journal of Agricultural Science and Botany
ISSN: 2591-7897 | Volume 2
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
PLANT GENOMICS
AND PLANT SCIENCE
World Congress on
Shikha Khandelwal et al., J Agric Sci Bot 2018, Volume 2 | DOI: 10.4066/2591-7897-C2-005
ANTIMICROBIAL ACTIVITY OF MORINGA
OLEIFERA AND TERMINALIA ARJUNA LEAF
EXTRACTS AGAINST FUNGAL PATHOGENS
Shikha Khandelwal and SM Paul Khurana
Amity Institute of Biotechnology, India
Medicinal plants are rich sources of biologically active compounds to
combat various diseases. The present study evaluated antifungal activi-
ty of aqueous extracts in phosphate buffered saline (PBS, pH 7.2), potassium
phosphate buffer (PPB, pH 7.0), sodium phosphate buffer (SPB, pH 7.0) and
sodium acetate buffer (SAB, pH 5.2) and in organic solvents (ethanol, hex-
ane, dichloro methane, butanol, and methanol) with different polarity from the
plants, Moringa oleifera and Terminalia arjuna, selected on the basis of their
use in traditional medicine. The leaf extracts were analyzed qualitatively and
quantitively for phytochemicals and found having flavonoids, phenols, tan-
nins, steroids, glycosides, alkaloids saponins etc. The antimicrobial activity
was evaluated based on the zone of inhibition, in agar well diffusion assay
against three fungi (A. niger, T. rubrum and Fusarium sp.). Maximum antimi-
crobial activity was observed in extracts with PBS for M. oleifera against A.
niger (30±0.05 mm) followed by T.rubrum (27±0.03 mm) whereas T. arjuna
extract showed maximum activity against A. niger (30±0.05 mm). Similarly,
antimicrobial activity in organic solvents showed dichloro methane extract
of M. oleifera with maximum activity against A. niger (15±0.03mm) and T.
arjuna extract against A. niger (13±0.04 mm). The plant extracts least activity
against Fusarium sp. Minimum inhibitory concentration (MIC) for M.oleifera
showed an overall highest activity in PBS buffer @ 24.35±0.02 mg/ml against
A. niger and dichloro methane extract against A.niger (MIC 56.26±0.05 mg/
ml) whereas T. arjuna extract registered maximum activity against A. niger
with MIC 45±0.05 mg/ml in PBS buffer and 83±0.43 mg/ml in dichloro meth-
ane , respectively. These extracts proved to be having fungicidal effects, sup-
porting their traditional use.
Shikha Khandelwal obtained BSc (2005) degree from
MDS University, Ajmer and MSc in Biotechnology (Gold
Medalist, 2007) from Rajasthan agricultural Universi-
ty, Bikaner. She joined IBI Biosolutions Pvt. Ltd. (2007)
Panchkula, for one year project based on “Biotech Indus-
trial Training Programme 2007-08” organized by Biotech
Consortium India Limited, where she worked on various
aspects of Bioinformatics tools and techniques including
‘In silico drug designing, Programming Language (PERL)
and comparative sequence analysis’.
BIOGRAPHY