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N o v e m b e r 0 5 - 0 6 , 2 0 1 8 | P h i l a d e l p h i a , U S A

INTERNATIONAL OBESITY SUMMIT AND EXPO

DIABETES, NUTRITION, METABOLISM & MEDICARE

International Conference on

Joint Event on

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

alliedacademies.com

YEARS

LASER, OPTICS AND PHOTONICS

World Conference on

Obesity Summit 2018 & Diabetes Conference 2018 & Laser Photonics Conference 2018

Biomedical Research

ISSN: 0976-1683

Volume 29

Kazi Ishtiaq Ahmad et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C7-019

ANTI-HYPERGLYCEMIC ACTION OF

GYNURA

PROCUMBENS

IS PARTLY MEDIATED BY

INHIBITION OF CARBOHYDRATE DIGESTION

AND ABSORPTION IN THE GUT

Kazi Ishtiaq Ahmad, Mahbub Hasan, Limu Parvin, Afra Haque,

Kamol Krishna Nandy

and

JMA Hannan

Independent University, Bangladesh

ynura procumbens

leaf has been widely used as a traditional therapy for

diabetes all over the world. Present study aims to investigate effects of

G. procumbens

in sucrose digestion and absorption in the gut to explore the

anti-hyperglycemic activity of this plant. The dried-powder-leaves of

G. pro-

cumbens

were extracted with methanol. Sucrose malabsorption in GI Tract

was evaluated in 20-hrs-fasted Long Evan rats by determining the amount of

sucrose remaining in six different parts of gastrointestinal tract after sucrose

load (2.5 g/kg

b.wt

), with or without 500 mg/kg dose of

G procumbens

ex-

tract. For evaluation of disaccharides activity, the extract was fed to 20-h-fast-

ed rats. After 60 min, the rats were sacrificed, and the small intestines were

isolated and homogenized. The homogenate (20μl) was incubated for 60

min at 37 °C with 40 mmol sucrose. Disaccharides activity was calculated

by glucose converted from sucrose as mol-mg glucose/protein/h. When the

extract of

G procumbens

was administered simultaneously with the sucrose

load, the residual sucrose content in the gastrointestinal tract was increased

significantly (p<0.01), especially in the upper intestine at 30 min, in the whole

intestine as well as cecum at 1 and 2 h. At 4 h, sucrose was not detected in the

gut in both groups. When extract was supplemented with the glucose solu-

tion, the percentage absorption of glucose was decreased by 13-19% during

whole perfusion period (p<0.05). It inhibited disaccharides (sucrose) activity

significantly (p<0.05) in rats. The anti-hyperglycemic activities of

G procum-

bens

in rats are partly mediated via delaying intestinal carbohydrate digestion

and absorption.

Kazi Ishtiaq Ahmad has recently completed masters

of pharmacy, major in clinical pharmacy and molecular

pharmacology from East West University, Dhaka, Ban-

gladesh and completed bachelor of pharmacy from BGC

Trust University, Bangladesh. He has completed his M

Pharm research under the active supervision of JMA

Hannan in the field of diabetes.

kazilingkon@gmail.com

BIOGRAPHY