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Insights Nutr Metab 2017
Volume 1 Issue 3 Nutrition World 2017
Notes:
Page 52
September 11-12, 2017 Edinburgh, Scotland
15 th World Congress on
Advances in Nutrition, Food Science & Technology
Polycyclic aromatic hydrocarbon binding
characteristics of Lactobacillus rhamnosus
NRRL B-442
Sebnem Kurhan and Ibrahim Cakir
Novel Food Technologies Development-Application and Research
Center, Turkey
Lactic acid bacteria are the co-habitant group of
human intestinal microbiota. As intestinal tract is the
last stop of genotoxins, before incorporating into the
circulating system, these microorganisms play a key role.
Lactobacillus rhamnosus NRRL B-442 is the most known
probiotic which has a great potent to adhere intestinal
mucosal cells. This study is aimed to reveal the decrease
of sixteen polycyclic aromatic hydrocarbons (PAHs)
content by Lactobacillus rhamnosus using HPLC (High-
performance liquid chromatography) assay. At 0, 3, 6,
12 and 24 hour samples were centrifuged, supernatants
collected and freezed at -24°C until extraction. Liquid-
liquid extraction was applied and analyzed with HPLC-
DAD. During incubation, L. rhamnosus cells vitality
was checked using plate count method. The research
indicated, L. rhamnosus can keep alive and reduce
efficiently PAHs from artificially contaminated PBS
(Phosphate-buffered saline) time-dependently and the
most mutagenic compound of PAHs, Benzo[a]pyrene was
completely removed from the medium both 0h and 24h.
Biography
Sebnem Kurhan obtained her Bachelor’s Degree at Uludag University,
Turkey in Food Engineering and completed her Master of Science program
in Department of Food Engineering, Ankara University, Turkey. After a short
experience in private sector, she started to work as a specialist and she started
her PhD at Abant Izzet Baysal University, Turkey during 2013. She works
on “DNA-bioprotective effects of industrially important lactic acid bacteria”
in her thesis. She has worked as a Researcher in 9 national projects and
published 1 paper and gave 2 oral and 2 poster presentations as author in
different international congresses. She has been working as a Specialist in
Novel Food Technologies Development, Application and Research Center in
Abant Izzet Baysal University. She is using actively high performance liquid
chromatography (HPLC), gas chromatography, laser scanning confocal
microscope, flow cytometer and particle size analyzer.
Sebnem Kurhan et al., Insights Nutr Metab 2017