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Journal of Nutrition and Human Health | Volume 3
May 23-24, 2019 | Vienna, Austria
Joint Event
2
nd
International Conference on
Gastroenterology and Digestive Disor
ders
17
th
International Conference on
Nutrition and Fitness
&
PPARγ/Pgc-1α-Fndc5 pathway up-regulation in Gastrocnemius and Heart muscle of
exercised, branched chain amino acid diet fed mice
Navid Abedpour
1, 3
, Kamran Ghaedi
1, 2
and
Mohammad Hossein Nasr Esfahani
1
1
Royan Institute for Biotechnology, Iran
2
University of Isfahan, Iran
3
Islamic Azad University, Iran
B
ranched Chain Amino Acids (BCAAs), including Leucine,
Isoleucine, and Valine, are essential amino acids that
body is unable to synthesize and needs to be provided by the
diet. BCAAs play a significant role in energy homeostasis as
they are important for the maintenance of skeletal muscle.
A great proportion of BCAAs metabolism occurs in skeletal
muscle, where metabolism is regulated. In the present
investigation, we have attempted to address whether a
combination of BCAAs supplement consumption with
aerobic exercise could elaborate the expression of PPARγ,
Pgc-1α and Fndc5 genes and mitochondrial biogenesis in
gastrocnemius muscle and heart tissue of male C57BL/6
mice.
Thirty-six young male mice with an average weight of 16±2g
were selected. Mice were randomly assigned to 6 groups: 20
mg/mL of BCAAs consumption with simultaneous exercise-
training (20BCAAs/Ex), 60 mg/mL of BCAAs consumption
with simultaneous exercise-training (60BCAAs/Ex), exercise-
trained with no BCAAs consumption group (EX), 20 mg/
mL BCAAs without exercise-training (20BCAAs), 60 mg/mL
BCAAs without exercise-training (60BCAAs), and untrained
mice without BCAAs consumption (sed).
The findings showed 20BCAAs/Ex group significantly
increased Fndc5, PPARγ, Pgc-1α genes expression in
skeletal and heart muscles. In additional, circulating Irisin
levels in 20BCAAs/Ex group were increased (p<0.05).
Furthermore, we assessed the expression of mitochondrial
genes in gastrocnemius and heart muscles. BCAAs were
increased the expression of mtDNA transcription factor
A (Tfam), Cox4i1, a and b subunits of the mitochondrial
H+-ATP synthase (a-F1-ATPase, b-F1-ATPase) in both of
gastrocnemius and heart muscles. However, the maximum
enhancement was yielded when BCAAs at concentration
of 20 mg/mL was supplemented. Notably, we found
that Sirt1 mRNA was increased in 20BCAAs/Ex group,
the same as Fndc5 mRNA in gastrocnemius and heart
muscles. Interestingly, plasma urea and lactate levels were
significantly enhanced in 60 mg/mL BCAAs administrated
mice which performed exercised (p<0.05).
Speaker Biography
Navid Abedpour has acquired his MSc degree in the field of Nutrition
and Sport. Currently, he is the senior researcher on a project tackling
unraveling of the molecular mechanisms of several miRNAs, lnc RNA
and Circular RNA to progress in diabetic and inactivity mouse that
induce with advanced glycation end products diet in Royan Institute
(Genetics Lab).
e:
abedpoor.navid@yahoo.com