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academies
Volume 2
June 11-13, 2018 | London, UK
Neurology and Neuroscience
6
th
International Conference on
Anti-oxidative and anti-inflammatory protection induced by ranolazine in astrocytes in primary culture
Constanza Aldasoro
1
, Adrian Jorda
2
, Sol Guerra-Ojeda
2
, Martin Aldasoro
2
, Patricia Marchio
2
, Mª Dolores Mauricio
2
, José Mª Vila
2
and
Soraya L Valles
2
1
General Hospital of Castellon, Spain
2
University of Valencia, Spain
R
anolazine (Rn), a piperazine derivative, is indicated for
the treatment of refractory chronic stable angina. Late
sodium current (INa) amplitude is increased in different
pathologies, such as myocardial ischemia. Rn induces benefits
in cardiovascular, brain and other organs and systems. The
present work was designed to determine the Rn protective role
against inflammation and oxidative stress produced by Aβ1-42
in astrocytes in primary culture. Cells were incubated with Rn
(10−7 , 10−6 and 10−5 M) during 24 hours. ELISA technique
was used to assay pro-inflammatory mediators IL-1β and
TNF-α. The expression of PPAR-γ, Mn-SOD and Cu/Zn-SOD was
determined by western blot techniques. The presence of Rn
diminished IL-1β and TNF-α levels and increased PPAR-γ protein
expression. Furthermore, antioxidant protein expression,
Cu/Zn-SOD and MnSOD was significantly increased after Rn
addition in astrocytes in primary culture. Accordingly with our
results, Rn (at therapeutic concentrations) showed protective
effects, such as anti-inflammatory and anti-oxidant actions in
astrocytes in primary culture affected by Aβ1-42 toxic peptide
e:
constanza.aldasoro@gmail.com