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Volume 2
June 11-13, 2018 | London, UK
Neurology and Neuroscience
6 th International Conference on
Cuprisone-dependent reactive changers of Central Nervous System in the mice and their Improvement
by Cytokine
Nataliia O Melnyk, Irina F Labunets, Anzhela E Rodnichenko, Svitlana Yu Rymar and Nataliia A Utko
National O O Bogomolets Medical University, Ukraine
In this experimental work aimed at investigation of
morphological changers in organs of central nervous system
(CNS) and neuroprotective effects of recombinant human
leukemia inhibitory factor (rhLIF) on mice of different ages with
cuprizonemodel of the 129/Svmice at 3-5 and
16-17months of age, we assessedmotor andemotional activity,
brain antioxidant enzymes, T-lymphocytes and macrophages.
After staining of histological sections of the brain and spinal
cord in toluidin blue, we determined the percentage of neurons
with unmodified, moderate and severe structural changes. We
studied changers of nervous fibers of organs of CNS after used
methods of electron microscopy and morphometry. Cuprizone
was provide daily for 3 weeks. RhLIF was injected after 7-days
cuprizone diet, one administration daily, 50 μg/kg. Cuprizone
decreased the amounts of crossed squares and faecal boluses
in the mice of both age groups. RhLIF restored emotional
activity in thesemice, but more in youngmice. In the cuprizone-
treated mice of both age groups, the percentage of neurons
with severe changes in the brain and spinal cord was increased
and rhLIF the amounts of neurons with destructive changes
were reduced, being less pronounced in aged mice. In the brain
of cuprizone-treated mice of both age groups, the activity of
catalase and glutathione peroxidase inhibits. The percentage
of T-lymphocytes and active macrophages was increased in
cuprizone-treatedmice of both age groups. Decrease of amount
and activity of macrophages after injections of the rhLIF was
observed. LIF may be a perspective neuroprotective drug.
Speaker Biography
Nataliia O Melnyk is the Professor of the Histology and Embryology Department of
National O.O.Bogomolets Medical University, the Leading Research Scientist of the
Experimental Modeling Laboratory at the State Institute of Genetic and Regenerative
Medicine National Academy of Medical Sciences of Ukraine, Kyiv. Graduated
from Kyiv National Taras Shevchenko University in 1993, after an assignment she
worked as an engineer in the Institute of Molecular Biology and Genetics. During
2008- 2011, she worked as Deputy Head of the Department of Education and
Methodology of the National O.O.Bogomolets Medical University. She has more
than 270 scientific and methodological works, 5 patents of scientific research.
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