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August 23-24, 2018 | Paris, France
Neurology and Neurological Disorders
18th International Conference on
Journal of Neurology and Neurorehabilitation Research | Volume 3
During the study of the ternary complexes-TC: nucleic
acids - liposomes from zwitterionic lipids, in the presence
of a number of divalent metal cations- (Ca, Mg, Fe, Co, etc),
the author concluded about the uniqueness and widespread
prevalence of such complexes in the cell. They are more
labile than lipoplexes-complexes of cationic lipids with DNA,
in addition have a more diverse structure and are more
dynamic, capable of creating various organelle-like structures,
or contacts between organelles in eukaryotes. In addition, TCs
are not toxic to cells, unlike lipoplexes. The author suggested a
possible scheme for the formation of nuclear pores involving
liposomes from zwitterionic lipids and double-stranded DNA
or triple-stranded hybrids DNA /low molecular weight RNA
(lmw RNA), which, when untwisted in pore annuli, give one or
two chains of ssDNA. The thermo-stability of DNA/lmw RNA
triple helix is lower than the same sequence of DNA. That
specifies preferential attachment of three-stranded hybrids to
membrane vesicles.
The ssDNA in the pore annulus is the reason for the enhanced
transcription of the genes attached to nuclear pore what shown
only now, but we are written about that fact 30 years ago.
The ssDNA is attractive site not only for DNA polymerase
but also is target for beta -amyloid (βA) too. For example, βA
binding to ssDNA of nuclear pore prevents template activity of
enzymes on the ssDNA. The β -amyloids concentrating in area
ssDNA (pore annulus) result in the βA aggregates appearing.
Lipids environment (nuclear envelope) as it known support the
aggregation of βA. With time in pore annulus forms βA plaque,
which could prevent RNA transport from nucleus to cytoplasm.
Therefore, exist at least two unknown way of influence of
nuclear pores on the regulation of cells gene activity resulting
to Alzheimer’s disease.
Speaker Biography
Vasily Kuvichkin is a Senior Staff Scientist in Laboratory of Mechanisms of Reception,
Institute of Cell Biophysics of the Russian Academy of Sciences, Russia. His research
interests include biophysics, molecular biology, lipid-nucleic acids interactions,
spectroscopy (UV-VIS and fluorescence), microscopy (EM and fluorescent), nuclear
pore assembly, system biology, evolution, aging. He has received many awards such
as “Vanguard of Knowledge” by pharmacy company “Astra Zeneca” in the year 2012;
Winner of Grant of the European Science Foundation: “Epitope map” from University
College of Dublin, Ireland in the year 2011; Winner of Grant of the DAAD, Berlin, MDC-
Buch, Germany in the year 2009; Winner of Japan Society for Promotion of Sciences
grant by Shizuoka University, Japan. He has published many research articles and is an
active member of many International Conferences.
e:
Vasily Kuvichkin
Russian Academy of Sciences, Russia
The role of lipids-nucleic acids interactions in nuclear pores assembly and in
Alzheimer’s disease origin