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allied
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Journal of Materials Science and Nanotechnology | Volume 2
October 29-30, 2018 | London, UK
Nanomaterials and Nanotechnology
International Conference on
Nano fountain probe technology for
in vitro
single cell studies
Horacio D Espinosa
Northwestern University, USA
W
e present a broadly-applicable Nano fountain Probe
technology for single cell delivery and analysis using
biomolecules and functional nanoparticles. The nano fountain
probe is a scanning probe nano delivery tool which makes
use of on-chip fluid reservoirs and integrated microchannels
to deliver liquid solutions to sharp-aperture dispensing
tips. The unique tip geometry allows for both sub-100nm
nanopatterning on substrates for subsequent cell culture, as
well as direct biomolecular delivery inside cells with minimum
invasiveness. The spatial and force resolution of the atomic
force microscope are leveraged to control the probe with
nanometer and nano newton precision during nanopatterning
and
in vitro
transfection experiments. We begin by describing
nanopatterning capabilities and their application to cell
adhesionandnanomaterial-mediateddelivery studies, followed
by
in vitro
single cell transfection of biomolecules (DNA, RNA,
plasmids). In this presentation, an emphasis is placed on the
broad utility of the nano fountain probe as a nano delivery tool
with the goal of motivating future studies in cell biology. Direct-
write nanopatterning of several biomolecules and functional
nanoparticles using the nano fountain probe will be presented.
Examples include DNA and protein, as well as gold and drug-
coated diamond nanoparticles. Models of the deposition
process describing the effects of probe geometry, liquid
properties and patterning parameters on resolution will be
discussed. These models enable optimization of the patterning
process, resulting in sub-100nm resolution. The need for high
resolution delivery arises in nanoscale studies of protein and cell
functions such as the creation of adhesion templates, where for
example, protein clustering in cell focal adhesion occurs at 5 to
200nm length scales. Similarly, this resolution allows extremely
precise spatial control of dosing in nanomaterial-mediated
drug delivery studies. As an example, patterning drug-coated
diamond nanoparticles, in which the dosing is controlled
with yoctogram precision, will be presented. Direct
in vitro
transfection of functionalized nanoparticles and biomolecules
will be discussed. The transfection of fluorescently-labeled
diamond nanoparticles, on multiple cancerous and normal
cell lines, will be illustrated. Likewise, the temporal delivery of
proteins and RNA molecular beacons will be discussed in the
context of non-destructive cell analysis.
e:
espinosa@northwestern.edu