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Journal of Biomedical Research | Volume: 29
November 19-20, 2018 | Paris, France
Molecular Biology, Tissue Science and Regenerative Medicine
International Conference on
Joint Event
&
4th World Heart Congress
Potentiation of the anticancer activity of methylglyoxal by creatine supplementation
Manju Ray1,2, Anirban Roy1 and Aparajita Pal1
1Bose Institute, India
2Institute of Applied Science & Humanities GLA University, India
The creatine kinase (CK) system plays a key role in cellular
energy buffering and transport. Our group demonstrated
the progressive decrease of phosphocreatine, creatine and CK
upon transformation of skeletal muscle into sarcoma. It was
convincingly revealed that prominent expression of creatine-
synthesizing enzymes L-arginine: Glycine amidinotransferase
and N-guanidinoacetatemethyltransferase occurs in sarcoma,
ehrlich ascites carcinoma and sarcoma 180 cells; Whereas,
both these enzymes were virtually undetectable in skeletal
muscle. Simultaneously, our group has been investigating the
anticancer activity of the glycolytic intermediate, methylglyoxal.
We observed that the tumor inhibitory effect of methylglyoxal
was significantly augmented in presence of creatine. Moreover,
creatine and CK, which were very low in sarcoma tissue, were
significantly elevated with the concomitant regression of
tumor. In recent research we verified that the potentiation of
the anticancer activity of methylglyoxal by creatine extends
to breast carcinoma model as well and formulated a creatine
supplemented methylglyoxal based anticancer formulation.
Our recent focus has shifted in combating the drug resistant
cancer stem cells, which is a major limitation of present day
cancer therapy. Unpublished data from our laboratory has
revealed the preferential anti-stem cancer cell activity of
methylglyoxal in breast carcinoma model. Methylglyoxal at
metronomic concentrations targets and reduces the population
of CD44hi/CD24lo breast cancer stem cells. As future research,
we aim to evaluate the potential of creatine supplementation in
intensifying the potency of methylglyoxal to suppress stemness
in cancer cells.
Speaker Biography
Manju Ray is an Indian scientist in Molecular Enzymology and Cancer Biochemistry. She
has done notable work in the development of anticancer drug and understanding of
differentiation process of cells. Her interests cover tumor biochemistry and molecular
enzymology.RaygraduatedfromtheCalcuttaUniversitywithdegrees inPhysiology
in 1969 and PhD in Biochemistry in 1975. She started her career in the Department of
Pharmacy, Jadavpur University and then shifted to Department of Biological Chemistry,
Indian Association of Cultivation of Science, India and became a professor. She is now
a Hon. Visiting Scientist at Bose Institute, India and also Distinguished Professor, GLA
University Mathura. Her research has, over a long span of her career in the department
of biochemistry at the Indian Association for the Cultivation of Science (IACS), Jadavpur, in
association with a team of scientists and doctors that has led to positive development of
a drug for cancer treatment. She is a Shanti Swarup Bhatnagar awardee (Highest honor in
science in India).
e:
Manju Ray et al., Molecular Biology & Heart Congress 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C8-022