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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4
th
International Conference on
Detection of colonization-infection by multi-drug resistant microorganisms in patients
with previous hospitalization
Konstantina Kontopoulou
1
, Sofia Zotou
1
,Chrysoula Belai
1
, Dimitra Doumpala
1
, Irene Koutsiouki
2
and
Poulcheria
Zagka
1
1
Department of Microbiology, G.Gennimatas General Hospital, Greece
2
NR Nurses, General Hospital Papageorgiou, Greece
Objective:
Detection of rectal and pharyngeal colonization by
multi-drug resistant microorganisms (MDR) in patients with
previous hospitalization in other hospitals.
Material-methods:
564 patients admitted toour hospital with
prior hospitalization in other hospitalswere screened forMDR
microorganisms by rectal and pharyngeal swabs within 24h of
their admission. The study did not include ICU patients. Τhe
patients were monitored for the development of any signs of
possible infection.
Pseudomonas aeruginosa
,
Acinetobacter
baumannii
complex and
Klebsiella pneumoniae
resistant to
carbapenems, methicillin resistant
Staphylococcus aureus
(MRSA) and vancomycin resistant
Enterococcus spp
. (VRE)
were concerned as MDR. The swabs were directly inoculated
onto chromID CARBA prototype medium (bioMerieux,
Marcyl'Etoile, France). Identification and susceptibility testing
were performed by VITEK 2 automated system (bioMerieux,
Marcy l'Etoile, France). The MICs of imipenem, meropenem,
ertapenem, tigecycline, vancomycin and teicoplanin were
determined using E-tests (bioMerieux, Marcy l'Etoile, France)
following the Clinical and Laboratory Standards Institute
(CLSI) guidelines and interpretative criteria. Detection of
KPC and VIM resistance genes was done via combined-disk
tests using meropenem with and without phenylboronic acid
(PBA), EDTA or both, as recommended by EUCAST.
Results:
51 patients (9%) were colonized by one or two
MDR microorganisms. Particularly, 20 (3.5%) were colonized
with
K. pneumoniae
, 21 (3, 7%) with
A. baumannii
complex
and 10 (1,8%) with
P. aeruginosa
resistant to carbapenems.
All strains of
K. pneumoniae
were KPC. 4 (0,7%) patients
were colonized with MRSA and 7 (1, 2%) were colonized
with 2 MDR microorganisms. Cohorting was applied in all
patients. 10 colonized patients developed an infection during
their hospitalization with a microorganism with the same
resistant phenotype as the colonization strain. Table 1 shows
the rates of colonization and infection by the responsible
microorganisms, while Table 2 indicates the type of infection.
Table1: Patients with colonization and corresponding infections
A.baumanniicplx
P.aeruginosa K.pneumoniae
MRSA VRE
Colonization 21
10
20
4
-
Infection
3
2
5
-
-
Table 2: Type of infection
Bloodstream
infection
Urinary tract
infection
Wound Infection
A. baumanniicplx
2
1
-
P. aeruginosa
1
-
1
K. pneumoniae
2
2
1
Conclusions:
Screening of colonization by multi-drug resistant
microorganisms in patients with previous hospitalization in another
healthcare institution is considered necessary for the timely apply of
patients cohorting and strongly implementation of contact precautions
to prevent and limit the spread of multidrug-resistant microorganisms.
Speaker Biography
Konstantina Kontopoulou has done her master’s in public health from
University of Macedonia, Greece and doing her PhD at Aristotle University
of Thessaloniki, Greece. She is specialized in medical biopathology and
worked as a chief of microbiology department at Interbalkan Medical
center, Thessaloniki and now she is working as a senior registrar of
microbiology department at Gennimates general hospital, Thessaloniki.
She has attended many conferences and has marked her imprint of
research by winning awards under various categories. She also worked
as a sub investigator for various clinical trial and research projects. She is
currently an active member of various committees such as Medical Society
of Thessaloniki, Greek society for Infection Control, Hellenic Microbiology
Society.
e:
konstantinakontopoulou9@gmail.comKonstantina Kontopoulou, Microbiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-006