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O c t o b e r 1 9 - 2 0 , 2 0 1 8 | T o k y o , J a p a n
Materials Summit 2018 & Separation Summit 2018
Materials Science and Nanotechnology
|
Volume 2
ADVANCED MATERIALS AND POLYMER SCIENCE
SEPARATION TECHNIQUES
&
International Conference on
International Conference and Expo on
Joint Event on
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alliedacademies.comYEARS
Xindu Geng et al., Mater Sci Nanotechnol 2018, Volume 2
ONLINE MULTIPLE-DIMENSIONAL LIQUID
CHROMATOGRAPHY BY A SINGLE COLUMN
Xindu Geng
1
, Minghui Geng
2
and
Yongjun Lu
2
1
Northwest University, China
2
Xi’an Aolan Science and Technology Development Company, China
M
ultiple-dimensional liquid chromatography (mD-LC) has been a power
tool for substances in a complex sample, especially for fast separation
of biopolymers in small scale in proteomics and drug purification in industrial
scale. For an interface transfer between two columns, both offline and online
manners can be adopted, the former can operate large sample, but very
slowly; while the latter is very fast but only can transfer volume solution in
μL scale with accounting of 1/10 to 1/100 of the fraction from the previous
separation mode. A problem with the both is that it is hard simultaneously to
satisfying with high sample size, high resolution, and high speed (three high).
My presentation is to solve the request of the “three high” by employing the
combination of steady-region/migration-region optimization of two variable
theory of substance under gradient elution, a suitably sized chromatographic
cake, and a fully automatic multiple liquid chromatograph. A key point is that
the SR can be used as an operation space to expedite protein separation.
Buffer exchange, collection of fractions, pre-concentration of target proteins,
resampling, and so on, which are typically performed offline in hours or even
days, but it can be carried out in minutes or even seconds in this operation
space. A crude extract containing 20 % CBD was purified to purity of 99% by
online 3D-LC (RP/RP/RP) with only a single column in less 90 min.
Xindu Geng has graduated from Northwest Univer-
sity (NWU, Xi’an) and became a Faculty Member of
Department of Chemistry of NWU, and then a Faculty
Member University of Minnesota in 1982-1983. He is
the Visiting Professor of Purdue University separately
at Department of Biochemistry in 1982-1984 and De-
partment of Chemistry in 1995-1996, as well a Visit-
ing Professor of Chemistry Department of Creighton
University in 2001. He is the Director of Institute of
Modern Separation Science of Northwest University.
He has published more than 300 papers in reputed
journals.
xdgeng@nwu.edu.cnBIOGRAPHY