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Materials Science and Nanotechnology | Volume 2
May 21-22, 2018 | New York, USA
International Conference on
Nanoscience & Technology
D
eliveryofmultipledrugshasgainedconsiderableattention
in recent years especially for the repair and regeneration
of hard tissue as in the case of bone tumors. Various kinds
of ceramic nanoparticles (NPs) were studied as bone graft
substituents and drug delivery vehicles. Among them calcium
phosphate ceramic (CPC) NPs were much focused due to
their unique advantages such as biocompatibility, bioactivity,
affinity to biopolymers and high osteogenic potential. CPC
forms themajormineral component of the bone andhas been
extensively used as a carrier for delivery of small molecules,
growth factors and for tissue engineering and orthopedic
applications. Local delivery of chemotherapeutic agents is
preferred for the treatment of metastatic bone cancer in
comparison with systemic administration. Co-delivery of a
protein and an anti-cancer drug is a challenging approach in
bone tissue engineering as well as drug delivery. Our current
study focuses on developing model ceramic-polymer hybrid
NPs for the treatment of bone cancer. Ceramic core with
polymer corona hybrid NPs were fabricated and evaluated
for the combined delivery of an anti-cancer drug and a
protein. Hydroxyapatite NPs were loaded with model protein
bovine serum albumin (BSA) and subsequently coated with
poly(vinyl alcohol)-methotrexate (MTX) conjugate. The
synthesized hybrid NPs were extensively characterized by
various techniques such as IR, XRD, NMR, TEM and TGA.
The coating efficiency was found to be 10-17%. In vitro drug
release performed in phosphate buffer for 2 weeks showed
simultaneous release of both MTX (88%) and BSA (76%) in a
sustained manner. Human osteosarcoma (OMG-63) cell line
was used to check the cytotoxicity and cellular uptake of NPs.
The NPs were biocompatible and exhibited anti-proliferative
activity in a concentration-dependent manner. Moreover,
the NPs showed excellent cellular uptake as confirmed by
flow cytometry and fluorescence imaging.
Speaker Biography
Ram Prasad S received B. Pharmacy and M. Pharmacy degree from Madras Medical
College, Chennai and currently doing PhD under the guidance of Prof. A. Jayakrishnan
(Biotechnology) and Prof T.S. Sampath Kumar (Metallurgical andMaterials Engineering)
from Indian Institute of Technology Madras, Chennai, Tamilnadu, India. His current
research is development of bone void filling materials composed of ceramic polymer
hybrid nanoparticles for the delivery of therapeutic molecules.
e:
pharmacist.ramprasad@gmail.comSimultaneous delivery of the protein and anti-Cancer drug for the treatment of bone cancer using
ceramic-polymer hybrid nanoparticles
Ram Prasad S
Indian Institute of Technology Madras, India