icnst-2018-posters-accepted-abstracts

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Materials Science and Nanotechnology | Volume 2

May 21-22, 2018 | New York, USA

International Conference on

Nanoscience & Technology

Synthesis of biopolymer based platinum nanoparticles And 5-fluorouracil loaded Platinum

nanoparticles and evaluation of their anti-tumor potential employing Potato Tumor Bioassay model

Godugu Deepika

Osmania University, India

facile and green synthesis of platinum nanoparticles

(GKPtNP) using biopolymer, Gum Kondagogu (GK) as a

reducing, capping and stabilizing agent was developed. The

formation and stability of GKPtNP was confirmed by UV–

visible spectroscopy, SEM-EDAX, TEM, XRD, Zeta potential

analysis, FTIR, ICP-MS and stability studies. The formed

GKPtNP are well dispersed, homogenous and showed a

characteristic absorption peak at 201 nm, with a size of

2-4±0.50 nm and recorded negative zeta potential (−46.1

mV) indicating good stability. 5-fluorouracil (5FU), a known

anticancer drug, was loaded into the synthesized GKPtNP,

which leads to the development of a new combination

of nanomedicine (5FU-GKPtNP). The in vitro drug release

studies of 5FU-GKPtNP in pH 7.4 showed a sustained release

profile over a period of 120 mins, and the hemolysis studies

revealed that GKPtNP, 5FU and 5FU-GKPtNP were found

to be blood compatible. A. tumefaciens induced in vitro

potato tumor bioassay was employed for screening the

anti-tumor potentials of GKPtNP, 5FU and 5FU-GKPtNP. The

experimental results suggested, a complete tumor inhibition

by 5FU-GKPtNP at a lower concentration than the GKPtNP

and 5FU respectively. Further, the mechanism of antitumor

activity was confirmed by their interactions with DNA using

gel electrophoresis and UV-spectroscopic analysis. The

electrophoresis results revealed that the 5FU-GKPtNP totally

diminishes DNA and the UV-spectroscopic analysis showed

hyperchromic effect with red shift indicating intercalation

type of binding with DNA. Over all, the present study revealed

that the combined exposure of the nanoformulation (5FU-

GKPtNP) resulted in the enhanced antitumor effect on A.

tumefaciens induced potato tumor bioassay.