herbal-medicine-2017-posters-accepted-abstracts

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alliedacademies
Allied J Med Res 2017
Volume 1 Issue 2 Herbal Medicine 2017
September 01-02, 2017 London, UK
3rd International Conference and Expo on
Herbal & Alternative Medicine
Notes:
Page 38
Korean Scutellaria Georgi flavonoid extract
induces mitochondrially mediated apoptosis
in human gastric cancer AGS cells
Gon Sup Kim1, Venu Venkatarame Gowda Saralamma1, Ho Jeong
Lee1, Gyeong Eun Hong1, Hyeon Soo Park1, Silvia Yumnam1 ,
Suchismita Raha1, Won Sup Lee2, Eun Hee Kim3, Nak Ju Sung1 ,
Sang Joon Lee4 and Jeong Doo Heo 4
1Research Institute of Life science and College of Veterinary Medicine,
Gyeongsang National University, Gazwa, Jinju, Republic of Korea,
2College of Medicine, Gyeongsang National University, Chilam, Jinju,
Republic of Korea,
3Department of Nursing Science, International University of Korea,
Jinju, Republic of Korea,
4Gyeongnam Department of Environment Toxicology and Chemistry,
Gyeongnam Biological Resource Research Center, Korea Institute of
Toxicology, Jinju, Republic of Korea
Objective/Purpose: In the present study, the anticancer
effect of flavonoid extract from Korean S. baicalensis
Georgi (FSB) was investigated with the aim of elucidating
the underlying molecular mechanisms of the anticancer
effect of FSB on AGS human gastric cancer cells.
Materials & Methods: The flavonoid compounds were
extracted with 70% methanol from radix of Korean
Scutellaria bicalensis Georgi (Jinju, Korea). We got the
AGS cells from the Korea Cell Line Bank (Seoul, Korea). All
experiments used that AGS cells were seeded into 6-well
plates and stabilized for 24 h. The cells were then treated
with or without Scutellaria bicalensis. Cells were cultured
in RPMI1640 medium supplemented with 10% FBS, and
1% penicillin, streptomycin in a humidified atmosphere
of 5% CO2 at 37°C. Cell viability was determined using
MTT assay. Apoptotic cells were detected using a FITC
annexin-V apoptosis detection kit 1 (BD Pharmingen, San
Diego, CA, USA). And the levels of the apoptosis related
proteins expression were analyzed by Western blot.
Results: Treatment of AGS cells with FSB significantly
inhibited cell viability in a concentration‑dependent
manner. Furthermore, FSB significantly increased the
proportion of cells in sub‑G1 phase, and Annexin V
and Hoechst 33258 fluorescent staining confirmed the
apoptotic cell death. Furthermore, Western blotting
results identified that treatment of AGS cells with FSB
significantly downregulated the expression of caspase
family members, namely procaspases 3 and 9, and poly
(ADP‑ribose) polymerase (PARP), and subsequently
upregulated cleaved caspase 3 and cleaved PARP. It
was observed that FSB treatment significantly decreased
the mitochondrial membrane potential of AGS cells.
In addition, the ratio of the mitochondrion‑associated
proteins B cell lymphoma 2‑associated X protein and B
cell lymphoma extra-large was upregulated.
Conclusion & Discussion: The results of the present
study indicate that FSB significantly inhibits cell viability
and induces apoptosis in AGS cells via the mitochondrially
mediated intrinsic apoptotic signalling pathway.
FSB‑induced apoptosis was identified to be mediated
by caspase activation and triggered by the modulation
of Bcl‑2 family proteins. To the best of our knowledge,
the present study is the first to elucidate the underlying
molecular mechanism for the anticancer activity of FSB in
human gastric cancer AGS cells. Therefore, the present
study provides novel insights into the biological effects
of FSB, which may possess therapeutic potential for the
treatment of human gastric cancer.
Biography
Gon Sup Kim has completed his PhD from Seoul National University and
Postdoctoral Studies from University of Pennsylvania School of Veterinary
Medicine. He is the Director of Korea National Animal Bio-resources Bank,
Research Institute of Life Science and Professor of College of Veterinary
Medicine, Gyeongsang National University South Korea.
Gon Sup Kim et al., Allied J Med Res 2017