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allied
academies
Asian Journal of Biomedical and Pharmaceutical Sciences | Volume 8
March 26-27, 2018 | Orlando, USA
World Summit on
Healthcare & Hospital Management
&
International Conference & Exhibition on
Biologics and Biosimilars
Background:
Liver is a metabolically active organ responsible
for many vital life functions. Due to its important activities,
the liver is exposed to a number of insults and is one of the
body’s organs most subject to injury. In spite of tremendous
advances in modern medicine, there are hardly any reliable
drugs that protect the liver from damage and/or help in
regeneration of hepatic cell. It is, therefore, necessary to
search for effective and safe herbal drugs for the treatment
of liver disease to replace currently used drugs of doubtful
efficacy and safety.
Aim:
The aim of this study was to investigate the
hepatoprotective activity of aqueous extract of
Nigella sativa
seed in highly active antiretroviral therapy (Lamivudine,
Zidovudine and Efavirenz) administered rats.
Materials & Methods:
Sixty rats weighed between 150-
200g were randomly divided into six groups and each group
comprised of ten rats. Rats in group I were administered
with distilled water. Rats in group II were administered with
highly active antiretroviral therapy only. Rats in groups III -
VI were administered 100, 200, 400 and 800mg/kg
Nigella
sativa
plus highly active antiretroviral therapy respectively.
The treatments were given orally for 28 consecutive days. On
the 29
th
day, all rats were sacrificed under light diethyl ether
anesthesia; blood samples were collected for the assessment
of biochemical parameters, while liver tissue was used for
histopathological assessment.
Results:
Serum levels of liver enzymes ALT, AST, ALP, and
GGT were significantly (p<0.05) increased and albumin
concentration was significantly decreased in animals treated
with highly active antiretroviral therapy as compared to the
normal control. Histopathological observations also revealed
severe damage in the structure of liver tissue in animals
administered with highly active antiretroviral therapy.
Treatment of highly active antiretroviral therapy exposed
animals with
Nigella sativa
showed marked improvement in
both biochemical and histopathological findings. Rise in liver
enzymes was almost restored to normal in animals treated
with
Nigella sativa
.
Conclusion:
Nigella sativa
through its antioxidant activity
effectively protects highly active antiretroviral therapy
induced liver toxicity.
e:
kissimudiey@yahoo.comHepatoprotective activity of aqueous seed extract of
Nigella sativa
against highly active antiretroviral
therapy induced hepatotoxicity in rats
Kissi Mudie
1
, Daniel Seifu
2
, Feyissa Challa
1
, Abiy Abebe
1
, Asfaw Debella
1
and
Atsbeha Gebregzabher
1
1
Public Health Institute, Ethiopia
2
Addis Ababa University, Ethiopia