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allied
academies
Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
Public health concerns of
Legionella pneumophila
in District Faisalabad
Adnan Mehmood, Muhammad Shahid Mahmood, Mashkoor Mohsin Gilani, Zia-ud-Din Sindhu, Faisal Siddique
and
Tajamal Irshad
University of Agriculture Faisalabad, Pakistan
L
egionellosis is one of the most life-threatening waterborne
disease round the globe.
Legionella pneumophila
serogroup-1 is the causative agent of this night mare. In
developing countries like Pakistan this disease remain
underdiagnosed and underreported mainly due to the lack
of clinical awareness, high cost of its diagnosis and technical
reasons. The present study was conducted with the main
objective of the isolation, identification and characterization
of
Legionella pneumophila
(
L. pneumophila
) from clinical and
water sources. For this purpose, a total of one hundred and
sixty-two samples including sputum, swab and water samples
were collected from different hospitals in district Faisalabad.
Legionella in water samples was concentrated through
filtration assembly by using a 0.22µm pore size filter paper
and then heat treated at 50
0
C for selective inhibition of non-
Legionella species. All samples were cultured on four different
media plates Staph 110, Salmonella Shigella Agar, MacConkey
agar and GVPC selective media (buffered charcoal yeast
extract agar with supplements). Cultural and morphological
characteristics of colonies of bacteria were observed daily for
seven days. Identification of the isolatewas done bymicroscopy
and biochemical tests. Nineteen isolates were positive for
L. pneumophila
analyzed by sodium hippurate hydrolysis
test. Antibiotic sensitivity pattern against pure isolates were
analyzed by the Kirby-Bauer’s method. Greyish white colonies
of
L. pneumophila
were resistance to ampicillin, amoxicillin and
erythromycin while yellowish green isolates were resistance
to ampicillin and gentamicin. All the isolated strains were
sensitive to chloramphenicol, tetracycline and ciprofloxacin
Speaker Biography
Adnan Mehmood from Pakistan has completed his MPhil in Microbiology degree under
the supervision of Muhammad Shahid Mahmood, Associate Professor, Institute of
Microbiology in University of Agriculture Faisalabad. Adnan is proficient in using variety
of lab equipments, isolate, identify organism from sample and implement measures to
eradicate contamination and aware in depth about every type of microbiological test and
knows how to control quality of products.
e:
adnanmehmod@yahoo.com