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Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
In vitro
regeneration of Pomegranate cv. Bhagwa through axillary and adventitious bud proliferation
Prabhuling Guranna, Rashmi H, Kulapathi H, Babu AG
and
Satish D
University of Horticulture Sciences, India
P
omegranate (
PunicagranatumL.
) is one of theoldest known
fruit crops of the tropics and subtropics. It is regenerated
through tissue culture directly by axillary bud or by callus
mediated adventitious bud proliferation. But both the methods
have pros and cons. The choice of the method specifically
dependsuponpurpose, quality, economy, potency, andduration
of the protocol. Keeping these facts in view, the present studies
were carried out to optimize the protocol for rapid and efficient
in vitro
regeneration of Pomegranate cv. Baghwa. In axillary bud
proliferation different explants, duration of mercuric chloride
treatment, antioxidants and growth regulators were tried for
improvising aseptic culture establishment. Among the various
treatments, surface sterilization of double nodal explants
containing IIIrd + IVth nodes with HgCl
2
0.10 % for 3 minutes
resulted in significantly better aseptic culture establishment (55
% aseptic culture, 15 % each bacterial contamination, fungal
contamination and phytotoxicity) onto MS medium containing
BAP 1 mg/l + AgNO
3
1 mg/l + activated charcoal 2000 mg/l.
Superior shoot proliferation (5 number of shoots/explants,
4.97 cm length of shoot and 18.23 number of leaves/shoot)
was found onto the MS medium containing ancymidol 0.02
mg/l + AgNO
3
1 mg/l + activated charcoal 500 mg/l. Among
the various media combination, effective rooting (22 number
of days taken for rooting, 48 % rooting, 4.30 cm length of roots
and 5.50 number of roots/shoot) was observed on half strength
MS medium supplemented with IBA 2 mg/l +AgNO
3
1 mg/l +
activated charcoal 200mg/l. Calluswas inducedwith exogenous
application of plant growth regulators for adventitious bud
proliferation. The nodal segment was found superior for
induction of callus (++++: Very good) when cultured onMS basal
medium consisting of BAP 5 mg/l + NAA0.4 mg/l. Early shoot
initiation (17.06 days), a greater number of shoots per explant
(8.13) and maximum shoot length (7.32 cm) was noticed when
proliferated calli were cultured onMS basal medium containing
BAP 2 mg/l + NAA 0.1mg/l + GA3 0.5 mg/l. Early
in vitro
root
initiation (20.25 days), highest per cent rooting (72.50) and
maximum number of roots per plantlet (3.95) were recorded
on full strength MS medium supplemented with IBA 3 mg/l.
Speaker Biography
Prabhuling Guranna has completed his PhD in Horticulture with specialization in banana
plant tissue culture in 2011 from University of Agricultural Sciences, Bangalore, India. He
participated in post graduate course on “Adapting to Climate Change: Biotechnology in
Agriculture in a World of Global Environmental Changes” from 2.05.2011to 30.06.2011 at
Rehovot, Israel. Presently he is working as Associate Professor of plant biotechnology at
University of Horticultural Sciences, Bagalkot, India. He has over 35 research publications
that have been cited over 12 times, his RG score is 9.11 and H-index is 2 and has been
serving as an editorial board member of reputed Journals viz., Research Journal of
Biotechnology and European Journal of Medicinal Plants. He is MASHAV alumni, life
member of International Society of Biotechnology, Karnataka Horticultural Society and
Association for the Improvement in Production and Utilization of Banana. He received
first best oral presentation award at National Conference on Production of Quality
Seeds and Planting Material – Health Management in Horticultural Crops in 2010.
e:
gprabhuling@gmail.com