food-science-dubai-2019-keynote

5 / 14

allied
academies
Page 12
Notes:
Journal of Food Science and Nutrition | Volume 2
December 09-10, 2019 | Dubai, UAE
Nutrition, Food Science and Technology
8th International Conference on
J Food Sci Nutr, Volume:2
The cytotoxic effects of amygdalin natural product on
cultures of breast cancer cells were investigated in vitro.
We used the cell lines MCF-7 and T47D, which are derived
from luminal A subtype of breast tumors carcinoma. Though
they vary in some molecular properties, these tumor cells
share the presence of positive estrogen, progesterone
receptors and lack of human epidermal growth factor 2.
Our data demonstrated a growth suppression of MCF-7 and
T47D by amygdalin in concentration and time-dependent
manners. This growth suppression was concomitantly linked
with an increase in the generation of malondialdehyde (MDA)
and oxidized glutathione together with a decline in the total
glutathione concentration and glutathione reductase activity.
The proportional cell survival of these tumor cells was
correlated positively with the total glutathione and inversely
with the amygdalin or MDA levels (p < 0.001). In MCF-7 cells,
the treatment with amygdalin showed 6 times less production
of total glutathione as compared to the untreated matched
tumor cells, whereas a similar amygdalin treatment of T47D
cells yielded only 2.1 times difference in total glutathione
generation between the amygdalin treated and untreated
tumor cells. Similarly, the amygdalin treatment of MCF-7 cells
exhibited 2.4 times higher production of MDA than in the
untreated tumor cells, while suchdifference inMDA formation
between the amygdalin treated and untreated T47D tumor
cells was dropped to 1.3 times. These data support an in vitro
mechanism of amygdalin antitumor action against breast
cancer cells potentiated by the induction of oxidative stress.
The cells ofMCF-7 originated froma highly proliferating breast
cancer tumors seem to be more vulnerable to the oxidative
stress mediated amygdalin cytotoxicity than T47D cells which
derived from a slowly proliferating breast cancer tumor.
Speaker Biography
Muayad M Abboud has done PhD in clinical biochemistry, Medical and
biological school, University of Southampton, England, followingly his
Postdoctorate Fellow in medical research unit, University of Sussex,
Brighton, England.
e:
Muayad M Abboud
Hashemite University, Jordan
Antitumor action of Amygdalin on human breast cancer cells
through selective sensitization to oxidative stress