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Journal of Food Science and Nutrition | Volume 2
December 09-10, 2019 | Dubai, UAE
Nutrition, Food Science and Technology
8
th
International Conference on
J Food Sci Nutr, Volume:2
M
icroorganisms are closely associated with and
controlling various metabolic activities directly or
indirectly in humans. The disruption and/or any disbalance
in the microbial composition may be linked to different
metabolic disorders. The chemotherapeutic treatments
used to cure the diseases are also known to pose negative
health impacts; also the development of antibiotic
resistance among pathogens has challenged or limited
their applications to control infections. Therefore, there
is a need to discover natural means to prevent and cure
the infections and other metabolic disorders. Probiotics
intervention as supplements or consumption of probiotics
carrying food products is one such promising approach that
can help maintain microbial homeostasis; thereby lead to
healthy life. In our lab, Lactobacillus reuteri strains from
breast fed infant feces were isolated and screened for various
probiotic attributes. Among various strains tested, L. reuteri
LR6 showed maximum tolerance to simulated gastric and
duodenum conditions, auto-aggregation, adhesion to Caco-
2 cell lines. The strain showed cholesterol lowering and
pathogen inhibition abilities under both in-vitro and in-vivo
conditions. Also, it was evident that Cell surface proteins
play an important role in probiotic activities, such as survival
in intestinal conditions, adhesion etc., of the strain LR6. The
cell surface proteins and extracellular proteins from the strain
LR6 were also found to play an important role in pathogens
inhibition and controlling the expression of various genes
involved in gut barrier functions using Caco-2 cell lines. It is
believed that the indigenous strain L. reuteri LR6 has great
potential and can be explored further for practical application.
Speaker Biography
Tejinder Pal Singh has completed his PhD at the age of 26 years from
National Dairy Research Institute, Karnal, India. He is working as an
Assistant Professor in Dairy Microbiology Department, College of Dairy
Science and Technology, LUVAS, Hisar, India. He has published over 15
research and review articles, 6 popular articles, 4 book chapters that
have been cited over 150 times. He has also received best paper and best
poster awards at different platforms.
e:
tejinder.codst@luvas.edu.inTejinder Pal Singh
Lala Lajpat Rai University of Veterinary and Animal Sciences, India
Probiotics intervention: A promising approach to control Metabolic
disorders and infections