euro-gastroenterology-clinical-pharmacy-2019-tracks

14 / 29

Euro Gastroenterology 2019 & Clinical Pharmacy 2019 Archives of General Internal Medicine | ISSN: 2591-7951 | Volume 3
Page 35
March 25-26, 2019 | Amsterdam, Netherlands
&
GASTROENTEROLOGY AND HEPATOLOGY
4th International Conference on
CLINICAL PHARMACY & PHARMACY PRACTICE
9th World Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
THE HISTOPATHOLOGICAL STUDY OF“HORSE BONE CALCIUM”PREPARATION
EFFECT ON THE ACETIC INDUCED GASTRIC ULCER MODEL
B Myagmarnaran 4, M Ariunaa1, Z Ariunaa2 , D Аdilzaya3 , B Altanshagai5 and
D Demberelnyamba 1
1 Science, Technology & Production “Monchemo” Co. Ltd., Mongolia
2 Treatment, Research and Production Company of The Mong-Em, Mongolia
3 Mongolian National University of Medicinal Sciences, Mongolia
4 Mongolian University of Pharmaceutical Science, Mongolia
5 New Medicine Medical University of Mongolia, Mongolia
Introduction: Calcium homeostats is: Horse bone calcium supplement contains calcium hydroxyapatite,
which is in the form of complex chelates ion, gives effect of diminish gastric acid and pepsin. Calcium hy-
droxy-apatite is crystallizes in the hexagonal crystal system. Size of the calcium hydroxy-apatite nano particles
are 105-120 nm.
Microcrystalline hydroxyapatite concentrate (MCHC) is derived from whole bone and is available as a nutri-
tional preparation. It provides much greater nourishment than just calcium. MCHC contains protein and other
ingredients that comprise the organic portion of bone, as well as calcium and other minerals in the normal
physiological proportions found in raw bone.
There is no doubt that calcium is essential for healthy bone formation; however, trace minerals and organic
factors are also important. Because bone is a complex, highly mineralized tissue, a number of trace mineral de-
ficiencies can impair bone formation and remodeling. Trace minerals also act as cofactors for several enzymes
involved in the production of the organic portion of bone. Because MCHC is actual bone, it contains these
vital components, which are important for a healthy skeleton. It truly is comprehensive bone nourishment. No
stress for gastric digestion system.
Materials and methods: The effects of the medicinal substances were investigated on“Wistar”breed of white
rats. Many studies mostly experimental animal effect have been conducted in thus field. In this study, we ex-
amined gastroprotection effect of “Horse bone calcium” preparationon the gastric ulcer in rats model induced
by acetic acid. The stomach exposed and 0.02 ml of 100% acetic acid was injected into the gastric wall near
the antral portion of the stomach using a hamilton syringe with a 30-gauge needle. Intact animals received no
surgical modifications. The abdomen was then sutured, and the animals were allowed to recover and returned
to their cages with food and water ad libitum. Injection of 0.02 ml of acetic acid causes. The stomach protective
process starts around day 7 and is completed in about 4 weeks.
The animals were divided into 3 groups and treated daily by gavage with “Horse bone calcium” in 1.2 ml of
solvent. At the end of 4 weeks, the animals were killed and their stomachs removed, opened along the greater
curvature, fixed in 10% neutral buffered formalin overnight, and photographs taken.
Results: Histopathological studies: Freshly excised stomach of one animal from each group was washed with
B Myagmarnaran et al., Arch Gen Intern Med 2019, Volume 3 | DOI: 10.4066/2591-7951-C1-023