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Euro Gastroenterology 2019 & Clinical Pharmacy 2019
Archives of General Internal Medicine | ISSN: 2591-7951 | Volume 3
Page 31
March 25-26, 2019 | Amsterdam, Netherlands
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GASTROENTEROLOGY AND HEPATOLOGY
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COMPARISON OF QUANTITATIVE HEPATITIS B VIRUS DNA REAL TIME PCR
(RT-PCR) WITH REVERSE TRANSCRIPTION PCR (RT-PCR)
Rubi Ghazala
Lahore General Hospital, Pakistan
Background:
Serum HBV DNA is a useful and reliable marker to diagnose and monitor HBV infection. The lim-
itation of HBV DNA is that it is expensive and that the assays lack uniformity and standardization. Hence there is
a need for more economical and reliable marker. HBsAg quantitation is one such surrogate serological marker.
The objective of the current study is to compare the serum hepatitis B virus DNA quantitative Real Time PCR with
Hepatitis B reverse transcription PCR (rt-PCR).
Methods:
Patients with HBV attending to the outpatient clinic of all departments were enrolled in the study.
Patients with undetectable HBV DNA levels and those co-infected with HCV or HIV were excluded from the study.
All patients were tested for serological markers like HBsAg, HBeAg, and HBV DNA-PCR. HBsAg quantification was
done using conventional ELISA immunoassay. Chi-square was used to compare between HBV DNA (RT-PCR) and
(rt-PCR) quantitation. Statistical analysis was done using SPSS and P value of <0.05 was considered significant.
Results:
A total of 661 patients were enrolled in the study. Out of 373 serum samples were analyzed by HBV RT-
PCR while 281 by HBV rt-PCR. 38.9% were females in group of HBV RT-PCR while, 32.7% in group of HBV rt-PCR
and mean age of patients in the entire study group was 33.01 years in group of HBV RT-PCR while, 34.61 years in
group of HBV rt-PCR. The mean ALT level was 57.6 U/L in group of HBV RT-PCR while, 51.00 in group of HBV rt-
PCR. 16.5% (n=61) in group of HBV RT-PCR while, 8.9% (n=33) in group of HBV rt-PCR were HBeAg positive. 94.9%
(n=351) in group of HBV RT-PCR while, 73.2% (n=271) in group of HBV rt-PCR were HBsAg positive. Mean HBV
DNA Positive 44.3% in group of HBV RT-PCR while, 14.6% in group of HBV rt-PCR. HBV DNA (positive) levels were
significantly higher in HBV RT-PCR patients compared with HBV rt-PCR patients (164 versus 54; p=0.001). Neither
HBsAg levels nor HBeAg levels were significant (p=0.573, 0.057). HBV Real Time RT-PCR is best for diagnosis of
HBV DNA PCR. Clinical significant result obtained from such test. HBV RT-PCR has become a useful and important
technology for diagnosis of HBV DNA PCR, it must be used appropriately.
Conclusions:
There is a significant difference between HBV DNA Real Time PCR (RT-PCR) with HBV DNA reverse
transcription PCR (rt-PCR) patients with hepatitis B virus but not in HBsAg and HBeAg.
Keywords:
Hepatitis B Virus, Real Time PCR, reverse transcription PCR, HBsAg quantitation
Rubi Ghazala, Arch Gen Intern Med 2019, Volume 3 | DOI: 10.4066/2591-7951-C1-023