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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Notes:
Generation of cloned adult muscular pigs with myostatin gene mutation by genetic engineering
Xi-Jun Yin
1
, Jin-Dan Kang
1
, Seokjoong Kim
2
, Long Jin
1
, Qing Guo
1
, Xiaoxu Xing
1
, Meifu Xuan
1
, Guanglei Zhang
1
, Qirong Luo
1
, Yong Soo Kim
3
, Chengdu Cui
1
,
Wenxue Li
1
, Zhengyun Cui
1
and
Jin-Soo Kim
4
1
Yanbian University, China
2
ToolGen, Inc., South Korea
3
University of Hawaii, USA
4
Institute for Basic Science, South Korea
B
ecause skeletal muscle is the most economically valuable
tissue in meat-producing animals, enhancing muscle
growth in these species may enhance the efficiency of meat
production. Skeletal muscle mass is negatively regulated
by myostatin (MSTN), and non-functional mutations of the
MSTN gene in various animal species have led to dramatic
hypermuscularity. This study was designed to assess
the characteristics of male MSTN-knockout (KO) pigs. A
transcription activator-like effector nuclease (TALEN) pair
targeting exon 1 of the swine MSTN gene was constructed
and used to transfect porcine fetal fibroblasts (PFFs). We
obtained a cell line that consisting of a 2-bp deletion in one
allele and a 4-bp deletion in the other allele, was used as
a donor to generate cloned pigs via SCNT, and delivered 18
live piglets. They developed and grown normally to sexual
maturity. These MSTN-KO boars grew normally to adulthood
and showed visually-clear hypermuscular characteristics,
increased carcass dressing percentage and loin eye size, and
decreased in backfat thickness. These pigs may show greater
meat production, as well as being used in animal models of
human diseases.
Speaker Biography
Xi-Jun Yin is working as the Director of Jilin Provincial Transgenic Animal and Embryo
Engineering Laboratory at Yanbian University. His research goal is to increase
reproductive efficiency of swine and to expand the genetic potential present in pig
embryos. Recently, his research team successfully produced myostatin gene knockout
double-muscled adult pigs.
e:
yinxj33@msn.com