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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
I
nfertilitynowadays is agrowinghealth issue in thedeveloped
world meaning that every year more and more couples visit
an assisted reproduction (ART) centre. However, the success
rate of the process is stagnating at about 30%. An effort is
made worldwide to find new additional indicators of embryo
viability to implement the routinely used morphological
evaluation. Spent embryo culture medium samples (n=201)
were measured using liquid chromatography coupled mass
spectrometry in a series of retrospective, blind experiments.
No sample preparation was made, 10 µl of sample was
directly injected into the instrument after the addition
of internal standard solution. A protein marker was found
which significantly (p<0.001) differed in quantity between
the samples of embryos which did, or did not implant.
This protein was identified as the alpha-1 chain of human
haptoglobin molecule. A significant correlation (p<0.001)
was also found when comparing the clinical outcome
(clinical pregnancy or no pregnancy) and the outcome
predicted by the measurements. The haptoglobin fragment
quantitation serves as an additional tool along the process
of morphological evaluation. The blind, retrospective results
provided a positive predictive value of more than 50%.
The negative predictive value of the analysis was 100%,
there were no embryos which were diagnosed as “viable”
but resulted in clinical pregnancy. The results provided a
contra selection tool, screening the embryos with good
morphological aspects, but no implantation potential.
e:
montsko.gergely@pte.huNon-invasivemass spectrometric viability assessment of
in vitro
fertilized embryos using the alpha-1 chain
of human haptoglobin
Gergely Montskó, Krisztina Gödöny, Ákos Várnagy, József Bódis
and
Gábor L Kovács
University of Pécs, Hungary
Notes: