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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Introduction:
The presence of high reactive oxygen species
(ROS) levels in semen is a major factor involved in the
decline of male fertility by impairing sperm motility and DNA
integrity. In semen, ROS are mainly produced by leucocytes
and abnormal sperm. We aimed to assess in situ the ROS
production in spermatozoa and leucocytes in the ejaculate of
infertile men using photometric nitroblue tetrazolium (NBT)
reduction assay.
Material & Methods:
38 semen samples from infertile
patients were investigated by semen analysis and stained
for ROS production with nitro blue tetrazolium. The
measurement of spermatozoa and leucocytes ROS generation
was evaluated via production of coloured formazan.
Results:
The mean value of positive staining NBT (NBT +) sperm
was 9.4% (2-35%). NBT (+) sperm rates were significantly
higher in the teratospermic group in comparison with the
normal morphology sperm group. We noted positive and
significant correlations between the levels of (NBT +) staining
sperm and some sperm morphological abnormalities:
cytoplasmic droplets, flagellar angulation and coiled tails.
Moreover, leucospermia was found in 28.9% of our samples
and we noted a significant negative correlation between
(NBT +) staining leucocytes in semen and typical morphology
spermatozoa rates.
Conclusion:
The NBT test is an inexpensive and easy-to-
perform assay that can be routinely applied to identify sperm
oxidative stress in infertile men. This assay can be used for
the evaluation of seminal leucocytes activation state. It can
also have significant clinical utility by the exploration of some
unexplained infertility and
in vitro
fertilization failures in
infertile couples.
e:
sellamiafifa@yahoo.frAssessment of reactive oxygen species production in semen by the nitroblue tetrazoliumreduction assay (NBT)
Afifa Sellami
1, 2
1
Medical School of Sfax, Tunisia
2
Hedi Chaker Academic Hospital, Tunisia