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S e p t e m b e r 2 0 - 2 1 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Dermatology Congress 2018 & World Nephrology 2018 Archives of General Internal Medicine | ISSN: 2591-7951 | Volume 2
2nd WORLD NEPHROLOGY AND THERAPEUTICS CONGRESS
DERMATOLOGY AND TRICHOLOGY
&
International Congress on
Burhanuldeen Husam Niyazi et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C5-014
ALCOHOL-FREE TOPICAL LIPOSOME AND
LIPOSOMAL GEL FORMULATIONS OF SOME
HORMONES
Burhanuldeen Husam Niyazi1, İsmail Aslan 2,3, Bekir Cakici3 and
Ali Fuat Aytekin3,4
1Niyazi Pharma, Iraq
2University of Health Sciences, Turkey
3SFA R&D Health Services Inc, Turkey
4Ageless Antiaging Clinic Institute, Turkey
Introduction: Liposomes are microscopic vesicles composed of membrane
like lipid layers surrounding aqueous compartments. The lipid layers are
made up mainly of phospholipids. Phospholipids are amphiphilic; they have a
hydrophilic head and a lipophilic tail. In aqueous solutions they are arranged
in bilayers, which form closed vesicles like artificial cells. Liposomes are
composed of small vesicles of a bilayer of phospholipids encapsulating
an aqeous space ranging from about 0.03 to 10 micron diameter. Physical
chemists, liposomes being well defined are one of the systems of choice to
study amphiphiles. Biophysicists, liposomes serve as the best model for
biological membranes. Biologists, liposomes serve as model to understand
organelles and their function and mechanism of action of complex systems.
Liposomes serve formedical and non-medical applications. Both are based on
the above. The success of liposomes for medical application is demonstrated
by 12 approved drugs including 2 vaccines. (Ambisome, Doxil, Daunoxome so
on). Gels are an excellent formulations for several routes of administration.
They are useful as liquid formulations in oral, topical, vaginal, and rectal
administration. There are many gelling agents. Some of the common ones
are Poloxamers and Carbopol derivatives . Testosterone is a steroid hormone
from the androgen group and is found in mammals, reptiles, birds, and other
vertebrates. In men, testosterone plays a key role in the development of male
reproductive tissues such as the testis and prostate as well as promoting
secondary sexual characteristics such as increased muscle and the growth
of body-hair. Appropriate testosterone therapy can prevent or reduce the
likelihood of osteoporosis, type 2 diabetes, cardio-vascular disease (CVD),
obesity, depression and anxiety and the statistical risk of early mortality.
Low testosterone also brings with it an increased risk for the development of
Alzheimer’s disease.
Aim of the study: The purpose of this study was the formulation of liposomal
gel formulations containing some hormones and hormone combinations and
determination particle size, zeta potential, FT-IR analysis, PLM, SEM using
different methods and to evaluate on studies. Herewith, we purposed to
overcome of problems some of the topical preparations containing alcohol
by preparing liposomal gel delivery systems.
Materials & Methods: Testosteron (TT) containing eight different liposome
formulations were prepared by using thin film, sonication and reverse phase
Burhanuldeen Husam Niyazi has completed his MSc
in Cosmetology fromYeditepe University, Turkey. He is
running a family pharmacy in Kirkuk, Iraq.
BIOGRAPHY
evaporation methods, respectively. Briefly,
liposome was prepared by dissolving the 40
μmolmL−1of phospholipids in30mL chloroform
in a round-bottom flask. The chloroform was
removedusing a rotary evaporator under reduced
pressure to form a thin film over the wall of the
flask. The dried film was then hydrated over
a water bath with distilled water. Free TT was
removed by centrifugation three times at 17,500
rpm for 45min for each of them. Then liposomes
were prepared by sonication process. The
liposomes were characterized by mean particle
size and size distribution, zeta potential. SEM
and PLM technique were employed for obtaining
size distribution and surface appearance and
lamellarity.
Results: During the study, mean particle size
and zeta potential of liposomes were analysed.
According to results, particle size of liposomes
were found as 676 nm±5.73, 908 nm±24.40,
1146 nm±12.30 and 674 nm±30.48, respectively.
According to SEM images, liquid state liposomes
were clearly showed not intact and having gaps
on the surface of bilayer structure. But, gel state
liposomes were obviously observed intact in
bilayer structure.
Conclusion: Generally, liposomes topical drug
applications are safer and less strict than the
intravenous applications. Liposomes are used
as a carrier for creams, gels (lipogelosome)
containing various herbal complexes or
essential oils, moisturizing agents, antibiotics,
and complex products containing recombinant
proteins for wound healing. These results
obtained in this study confirm that alcohol
free liposomal gel containing testosterone
formulations are relatively safe than the other
commercial testosterone products. Preliminary
studies supported that liposome gel delivery
system is suitable for topical applications.